SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePCL9
Systematic nameYDL179W
DescriptionPHO85 cyclin-9

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ12477
SGD YDL179W
Gene Ontology (GO)
Cellular componentGO:0000307cyclin-dependent protein kinase holoenzyme ...TAS:SGD.
Molecular functionGO:0016538cyclin-dependent protein kinase regulator a...TAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007049cell cycleTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length304
Molecular weight34798

	---------+----------+----------+----------+----------+
MISDYDALLQ FNKKPVSQEM IQFLATSTAS IIKIRENNNP IQGCRPPDLS   50
IFIKNVVIQS NVQTPTLMAT SVYLNKLKSV IPKNVYGINT TRHRIFLGCL   100
ILAAKTLNDS SPWNKHWTTY TEGLLRIREV NTIERELLEY LNWDVRITTP   150
DLIDSLSYFL GPIKEQLFLQ RRQEMLLFNA PSPGQLKEYI NHRRPVSHSR   200
TSSAISVPSL TSMATVSTTD SRSSLLAKYQ PSLPLVESDN FNKKNHVPLR   250
NNNDICNNFR AEENIHSVNH IDVTMGSSPV MSHKPTIHQR LNFTRRGWSS   300
FFKQ                                                     304