SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePCL1
Systematic nameYNL289W
AliasHCS26
DescriptionPHO85 cyclin-1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP24867
SGD YNL289W
Gene Ontology (GO)
Cellular componentGO:0000307cyclin-dependent protein kinase holoenzyme ...TAS:SGD.
Molecular functionGO:0016538cyclin-dependent protein kinase regulator a...TAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0007049cell cycleTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length279
Molecular weight32095

	---------+----------+----------+----------+----------+
MCEYSKALHI LLKSPVTDDI IKFLTDTTLR VVPSSNYPTP PGSPGEKHLT   50
RLPSLMTFIT RLVRYTNVYT PTLLTAACYL NKLKRILPRD ATGLPSTIHR   100
IFLACLILSA KFHNDSSPLN KHWARYTDGL FTLEDINLME RQLLQLLNWD   150
LRVNTEDLIL DLQPLLEPIK QDLARSSDQR KRINMMMSMN RRTCAGTSPI   200
RSNNRFKLYE KQRNVSIASD LSSATLVDSC NDLRRLKDVT NIANNTVANT   250
NYVRTVEKWN DNVNRQSWDL EQIMSQHGF                          279