SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePBS2
Systematic nameYJL128C
AliasHOG4, SFS4, SSK4
DescriptionMAP kinase kinase PBS2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP08018
SGD YJL128C
Gene Ontology (GO)
Cellular componentGO:0005935cellular bud neckIDA:SGD.
Cellular componentGO:0005934cellular bud tipIDA:SGD.
Cellular componentGO:0031416NatB complexIDA:UniProtKB.
Molecular functionGO:0004708MAP kinase kinase activityIMP:SGD.
Molecular functionGO:0005078MAP-kinase scaffold activityIPI:SGD.
Molecular functionGO:0004596peptide alpha-N-acetyltransferase activityIMP:UniProtKB.
Biological processGO:0007015actin filament organizationIMP:SGD.
Biological processGO:0000169activation of MAPK activity during osmolari...IMP:SGD.
Biological processGO:0006972hyperosmotic responseIMP:SGD.
Biological processGO:0017196N-terminal peptidyl-methionine acetylationIGI:UniProtKB.
Biological processGO:0000208nuclear translocation of MAPK during osmola...IMP:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:UniProtKB.
EC number 2.7.12.2

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
38Phosphoserine.
66Phosphoserine.
68Phosphoserine.
71Phosphoserine.
81Phosphoserine.
248Phosphoserine.
269Phosphoserine.
514Phosphoserine.
518Phosphothreonine.

Sequence Information
Sequence length668
Molecular weight72720

	---------+----------+----------+----------+----------+
MEDKFANLSL HEKTGKSSIQ LNEQTGSDNG SAVKRTSSTS SHYNNINADL   50
HARVKAFQEQ RALKRSASVG SNQSEQDKGS SQSPKHIQQI VNKPLPPLPV   100
AGSSKVSQRM SSQVVQASSK STLKNVLDNQ ETQNITDVNI NIDTTKITAT   150
TIGVNTGLPA TDITPSVSNT ASATHKAQLL NPNRRAPRRP LSTQHPTRPN   200
VAPHKAPAII NTPKQSLSAR RAVKLPPGGM SLKMPTKTAQ QPQQFAPSPS   250
NKKHIETLSN SKVVEGKRSN PGSLINGVQS TSTSSSTEGP HDTVGTTPRT   300
GNSNNSSNSG SSGGGGLFAN FSKYVDIKSG SLNFAGKLSL SSKGIDFSNG   350
SSSRITLDEL EFLDELGHGN YGNVSKVLHK PTNVIMATKE VRLELDEAKF   400
RQILMELEVL HKCNSPYIVD FYGAFFIEGA VYMCMEYMDG GSLDKIYDES   450
SEIGGIDEPQ LAFIANAVIH GLKELKEQHN IIHRDVKPTN ILCSANQGTV   500
KLCDFGVSGN LVASLAKTNI GCQSYMAPER IKSLNPDRAT YTVQSDIWSL   550
GLSILEMALG RYPYPPETYD NIFSQLSAIV DGPPPRLPSD KFSSDAQDFV   600
SLCLQKIPER RPTYAALTEH PWLVKYRNQD VHMSEYITER LERRNKILRE   650
RGENGLSKNV PALHMGGL                                      668