SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard namePAK1
Systematic nameYER129W
DescriptionSerine/threonine-protein kinase PAK1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP38990
SGD YER129W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0004672protein kinase activityIDA:SGD.
Biological processGO:0006261DNA-dependent DNA replicationIGI:SGD.
Biological processGO:0006006glucose metabolic processIGI:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
40Phosphoserine.
41Phosphoserine.
1126Phosphoserine.
1128Phosphothreonine.

Sequence Information
Sequence length1142
Molecular weight126872

	---------+----------+----------+----------+----------+
MDRSDKKVNV EEVNVPSNLQ IELEKSGTSS SVSLRSPTKS SATNLAGMAE   50
GARDNASIAS SSVDSLNMLL ERQRVRQLNH PQHQQHISSS LAKTPTTTSS   100
FCSSGSSKNK VKETNRISLT YDPVSKRKVL NTYEIIKELG HGQHGKVKLA   150
RDILSKQLVA IKIVDRHEKK QRKFFTFIKS SKISENDKIK REIAIMKKCH   200
HKHVVQLIEV LDDLKSRKIY LVLEYCSRGE VKWCPPDCME SDAKGPSLLS   250
FQETREILRG VVLGLEYLHY QGIIHRDIKP ANLLISGDGT VKISDFGVSL   300
AASSTNSSDS SESLDELELA KTVGTPAFFA PEMCLGEDAF TRYNLTKENL   350
FRGSCISFMI DIWAVGVTLY CLLFGMLPFF SDFELKLFEK IVNDPLKFPT   400
FKEIQSNKVS KVSCEEEYEM AKDLLLKLLE KNPQKRMTIP AIKKHPFVSW   450
DFDHVPENDE KLLSSVLEQK LRFQCNQTDQ FEPISISKHE LKNAVSGVGK   500
KIKESVLKSI PLKDPSDLSN KNYLHPTETT RGRGDANVIV SEGSVLSNIK   550
ELSANDGCLN TDSDTNININ DDDHYSGDDN DGHLTKRELE RELNKFDDKH   600
EAGNMVNLPI NSSFASLDSF YIDNFAMARM GMSSPEAGDS VSSVPNLPSA   650
PSSTRLGRSP VFSGVTNQPS PIRPVLPQQK SSFCATGRYD KSHNSLLRNS   700
SSHLTSYNSG RPSSRTGRMN SRNQNLPKIP NSLSKISTTK LTELRVPKDS   750
EIPSPAKNPN ADRLRRFPVK KNTKTPAIKD PPRININSSD KSGSKNSPIK   800
SLYQRMKQSK DNSKTFEVRR GNFFSHFNGD DDDSSSQSSV TSSGSESDSE   850
LSSTSSSCTS GTQSRNSSNN NAYSETESLP FEFGVDSEDG SGVLLRDLPN   900
EDQIRPFLDI QPCRRMKVKS SLNLEPPSVS SSSSSSSDED ELILNVGTAG   950
HRRRHNSSKL SELSNSPQKG SNNFMYSNGS VHDSETTITP QNMDDLTLHQ   1000
ALSRSQPISK PGPLVLPKRL DQKKATTETS NLTDIVEFNG NNDHRKDKNF   1050
DKVLYSRDLL KDALSSTNAG RRRSIPSNKI RGRKDASITM STNVGNDEHA   1100
RNTSCHGDKG QENGAIKQRT HERSRSLTVA ELNEEKRRSA LP           1142