SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameOST3
Systematic nameYOR085W
DescriptionDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP48439
SGD YOR085W
Gene Ontology (GO)
Cellular componentGO:0016021integral to membraneIMP:SGD.
Cellular componentGO:0008250oligosaccharyl transferase complexIPI:SGD.
Molecular functionGO:0004579dolichyl-diphosphooligosaccharide-protein g...IMP:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006487protein amino acid N-linked glycosylationIPI:SGD.
Biological processGO:0006461protein complex assemblyIMP:SGD.
EC number 2.4.1.119

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length350
Molecular weight39483

	---------+----------+----------+----------+----------+
MNWLFLVSLV FFCGVSTHPA LAMSSNRLLK LANKSPKKII PLKDSSFENI   50
LAPPHENAYI VALFTATAPE IGCSLCLELE SEYDTIVASW FDDHPDAKSS   100
NSDTSIFFTK VNLEDPSKTI PKAFQFFQLN NVPRLFIFKP NSPSILDHSV   150
ISISTDTGSE RMKQIIQAIK QFSQVNDFSL HLPMDWTPII TSTIITFITV   200
LLFKKQSKLM FSIISSRIIW ATLSTFFIIC MISAYMFNQI RNTQLAGVGP   250
KGEVMYFLPN EFQHQFAIET QVMVLIYGTL AALVVVLVKG IQFLRSHLYP   300
ETKKAYFIDA ILASFCALFI YVFFAALTTV FTIKSPAYPF PLLRLSAPFK   350