SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameOST1
Systematic nameYJL002C
AliasNLT1
DescriptionDolichyl-diphosphooligosaccharide--protein glycosyltransferase subunit 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP41543
SGD YJL002C
Gene Ontology (GO)
Cellular componentGO:0008250oligosaccharyl transferase complexIPI:SGD.
Molecular functionGO:0004579dolichyl-diphosphooligosaccharide-protein g...IDA:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Biological processGO:0018279protein amino acid N-linked glycosylation v...IPI:SGD.
EC number 2.4.1.119

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length476
Molecular weight54072

	---------+----------+----------+----------+----------+
MRQVWFSWIV GLFLCFFNVS SAAQYEPPAT WENVDYKRTI DVSNAYISET   50
IEITIKNIAS EPATEYFTAF ESGIFSKVSF FSAYFTNEAT FLNSQLLANS   100
TTAPGDDGES EIRYGIIQFP NAISPQEEVS LVIKSFYNTV GIPYPEHVGM   150
SEEQHLLWET NRLPLSAYDT KKASFTLIGS SSFEEYHPPN DESLLGKANG   200
NSFEFGPWED IPRFSSNETL AIVYSHNAPL NQVVNLRRDI WLSHWASTIQ   250
FEEYYELTNK AAKLSKGFSR LELMKQIQTQ NMRQTHFVTV LDMLLPEGAT   300
DHYFTDLVGL VSTSHAERDH FFIRPRFPIF GGWNYNFTVG WTNKLSDFLH   350
VSSGSDEKFV ASIPILNGPP DTVYDNVELS VFLPEGAEIF DIDSPVPFTN   400
VSIETQKSYF DLNKGHVKLT FSYRNLISQV ANGQVLIKYD YPKSSFFKKP   450
LSIACYIFTA LMGVFVLKTL NMNVTN                             476