SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameOLE1
Systematic nameYGL055W
DescriptionAcyl-CoA desaturase 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2 MULTI    RPN10 Proteasomal Degradation
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP21147
SGD YGL055W
Gene Ontology (GO)
Cellular componentGO:0005789endoplasmic reticulum membraneTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004768stearoyl-CoA 9-desaturase activityIMP:SGD.
Biological processGO:0006636fatty acid desaturationIMP:SGD.
Biological processGO:0000001mitochondrion inheritanceTAS:SGD.
EC number 1.14.19.1

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length510
Molecular weight58403

	---------+----------+----------+----------+----------+
MPTSGTTIEL IDDQFPKDDS ASSGIVDEVD LTEANILATG LNKKAPRIVN   50
GFGSLMGSKE MVSVEFDKKG NEKKSNLDRL LEKDNQEKEE AKTKIHISEQ   100
PWTLNNWHQH LNWLNMVLVC GMPMIGWYFA LSGKVPLHLN VFLFSVFYYA   150
VGGVSITAGY HRLWSHRSYS AHWPLRLFYA IFGCASVEGS AKWWGHSHRI   200
HHRYTDTLRD PYDARRGLWY SHMGWMLLKP NPKYKARADI TDMTDDWTIR   250
FQHRHYILLM LLTAFVIPTL ICGYFFNDYM GGLIYAGFIR VFVIQQATFC   300
INSLAHYIGT QPFDDRRTPR DNWITAIVTF GEGYHNFHHE FPTDYRNAIK   350
WYQYDPTKVI IYLTSLVGLA YDLKKFSQNA IEEALIQQEQ KKINKKKAKI   400
NWGPVLTDLP MWDKQTFLAK SKENKGLVII SGIVHDVSGY ISEHPGGETL   450
IKTALGKDAT KAFSGGVYRH SNAAQNVLAD MRVAVIKESK NSAIRMASKR   500
GEIYETGKFF                                                510