SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNUP2
Systematic nameYLR335W
DescriptionNucleoporin NUP2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32499
SGD YLR335W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0000790nuclear chromatinIDA:SGD.
Cellular componentGO:0005643nuclear poreIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0005198structural molecule activityTAS:SGD.
Biological processGO:0006348chromatin silencing at telomereIMP:SGD.
Biological processGO:0006406mRNA export from nucleusTAS:SGD.
Biological processGO:0006609mRNA-binding (hnRNP) protein import into nu...TAS:SGD.
Biological processGO:0006607NLS-bearing substrate import into nucleusTAS:SGD.
Biological processGO:0006999nuclear pore organization and biogenesisTAS:SGD.
Biological processGO:0006611protein export from nucleusTAS:SGD.
Biological processGO:0006610ribosomal protein import into nucleusTAS:SGD.
Biological processGO:0006407rRNA export from nucleusTAS:SGD.
Biological processGO:0006408snRNA export from nucleusTAS:SGD.
Biological processGO:0006608snRNP protein import into nucleusTAS:SGD.
Biological processGO:0006409tRNA export from nucleusTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
14Phosphothreonine.
17Phosphoserine.
20Phosphoserine.
33Phosphoserine.
68Phosphoserine, by CHK2.
84Phosphoserine.
142Phosphothreonine.
163Phosphoserine.
165Phosphoserine.
190Phosphoserine.
203Phosphoserine.
205Phosphoserine.
219Phosphoserine.
224Phosphoserine.
248Phosphoserine.
284Phosphoserine, by CHK2.
317Phosphoserine, by CHK2.
351Phosphoserine, by CHK2.
368Phosphoserine, by CHK2.
387Phosphoserine.
399Phosphoserine, by ATM or ATR.
439Phosphoserine.
494Phosphothreonine.
512Phosphoserine, by CHK2.
516Phosphoserine.
523Phosphoserine, by CHK2.
525Phosphoserine.
540Phosphoserine.
561Phosphoserine.
572Phosphoserine.
581Phosphoserine.
590Phosphothreonine.
618Phosphothreonine.

Sequence Information
Sequence length720
Molecular weight77881

	---------+----------+----------+----------+----------+
MAKRVADAQI QRETYDSNES DDDVTPSTKV ASSAVMNRRK IAMPKRRMAF   50
KPFGSAKSDE TKQASSFSFL NRADGTGEAQ VDNSPTTESN SRLKALNLQF   100
KAKVDDLVLG KPLADLRPLF TRYELYIKNI LEAPVKSIEN PTQTKGNDAK   150
PAKVEDVQKS SDSSSEDEVK VEGPKFTIDA KPPISDSVFS FGPKKENRKK   200
DESDSENDIE IKGPEFKFSG TVSSDVFKLN PSTDKNEKKT ETNAKPFSFS   250
SATSTTEQTK SKNPLSLTEA TKTNVDNNSK AEASFTFGTK HAADSQNNKP   300
SFVFGQAAAK PSLEKSSFTF GSTTIEKKND ENSTSNSKPE KSSDSNDSNP   350
SFSFSIPSKN TPDASKPSFS FGVPNSSKNE TSKPVFSFGA ATPSAKEASQ   400
EDDNNNVEKP SSKPAFNLIS NAGTEKEKES KKDSKPAFSF GISNGSESKD   450
SDKPSLPSAV DGENDKKEAT KPAFSFGINT NTTKTADTKA PTFTFGSSAL   500
ADNKEDVKKP FSFGTSQPNN TPSFSFGKTT ANLPANSSTS PAPSIPSTGF   550
KFSLPFEQKG SQTTTNDSKE ESTTEATGNE SQDATKVDAT PEESKPINLQ   600
NGEEDEVALF SQKAKLMTFN AETKSYDSRG VGEMKLLKKK DDPSKVRLLC   650
RSDGMGNVLL NATVVDSFKY EPLAPGNDNL IKAPTVAADG KLVTYIVKFK   700
QKEEGRSFTK AIEDAKKEMK                                     720