SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNUM1
Systematic nameYDR150W
AliasPAC12
DescriptionNuclear migration protein NUM1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionQ00402
SGD YDR150W
Gene Ontology (GO)
Cellular componentGO:0005938cell cortexIDA:SGD.
Cellular componentGO:0005934cellular bud tipIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0015631tubulin bindingIPI:SGD.
Biological processGO:0000226microtubule cytoskeleton organization and b...IMP:SGD.
Biological processGO:0000266mitochondrial fissionIMP:SGD.
Biological processGO:0000001mitochondrion inheritanceIGI:SGD.
Biological processGO:0030473nuclear migration, microtubule-mediatedIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2197Phosphoserine.
2220Phosphoserine.
2221Phosphoserine.
2468Phosphoserine.
2469Phosphothreonine.
2471Phosphoserine.
2516Phosphoserine.
2519Phosphoserine.
2743Phosphoserine.

Sequence Information
Sequence length2748
Molecular weight313033

	---------+----------+----------+----------+----------+
MSHNNRHKKN NDKDSSAGQY ANSIDNSLSQ ESVSTNGVTR MANLKADECG   50
SGDEGDKTKR FSISSILSKR ETKDVLPEFA GSSSHNGVLT ANSSKDMNFT   100
LELSENLLVE CRKLQSSNEA KNEQIKSLKQ IKESLSDKIE ELTNQKKSFM   150
KELDSTKDLN WDLESKLTNL SMECRQLKEL KKKTEKSWND EKESLKLLKT   200
DLEILTLTKN GMENDLSSQK LHYDKEISEL KERILDLNNE NDRLLISVSD   250
LTSEINSLQS NRTERIKIQK QLDDAKASIS SLKRKVQKKY YQKQHTSDTT   300
VTSDPDSEGT TSEEDIFDIV IEIDHMIETG PSVEDISEDL VKKYSEKNNM   350
ILLSNDSYKN LLQKSESASK PKDDELMTKE VAENLNMIAL PNDDNYSKKE   400
FSLESHIKYL EASGYKVLPL EEFENLNESL SNPSYNYLKE KLQALKKIPI   450
DQSTFNLLKE PTIDFLLPLT SKIDCLIIPT KDYNDLFESV KNPSIEQMKK   500
CLEAKNDLQS NICKWLEERN GCKWLSNDLY FSMVNKIETP SKQYLSDKAK   550
EYDQVLIDTK ALEGLKNPTI DFLREKASAS DYLLLKKEDY VSPSLEYLVE   600
HAKATNHHLL SDSAYEDLVK CKENPDMEFL KEKSAKLGHT VVSNEAYSEL   650
EKKLEQPSLE YLVEHAKATN HHLLSDSAYE DLVKCKENPD MEFLKEKSAK   700
LGHTVVSNEA YSELQRKYSE LEKEVEQPSL AYLVEHAKAT DHHLLSDSAY   750
EDLVKCKENP DVEFLKEKSA KLGHTVVSSE EYSELQRKYS ELEKEVEQPS   800
LAYLVEHAKA TDHHLLSDSA YEELVKCKEN PDMEFLKEKS AKLGHTVVSN   850
EAYSELEKKL EQPSLAYLVE HAKATDHHLL SDSAYEDLVK CKENSDVEFL   900
KEKSAKLGHT VVSNEAYSEL EKKLEQPSLA YLVEHAKATD HHLLSDSAYE   950
DLVKCKENPD MEFLKEKSAK LGHTVVSNEA YSELEKKLEQ PSLEYLVEHA   1000
KATNHHLLSD SAYEDLVKCK ENPDMEFLKE KSAKLGHTVV SNEAYSELEK   1050
KLEQPSLEYL VEHAKATNHH LLSDSAYEEL VKCKENPDVE FLKEKSAKLG   1100
HTVVSNEAYS ELEKKLEQPS LEYLVEHAKA TNHHLLSDSA YEELVKCKEN   1150
PDVEFLKEKS AKLGHTVVSN EAYSELEKKL EQPSLAYLVE HAKATDHHLL   1200
SDSAYEDLVK CKENPDVEFL KEKSAKLGHT VVSNEAYSEL EKKLEQPSLA   1250
YLVEHAKATD HHLLSDSAYE DLVKCKENPD MEFLKEKSAK LGHTVVSNEA   1300
YSELEKKLEQ PSLEYLVEHA KATNHHLLSD SAYEDLVKCK ENPDMEFLKE   1350
KSAKLGHTVV SNKEYSELEK KLEQPSLEYL VKHAEQIQSK IISISDFNTL   1400
ANPSMEDMAS KLQKLEYQIV SNDEYIALKN TMEKPDVELL RSKLKGYHII   1450
DTTTYNELVS NFNSPTLKFI EEKAKSKGYR LIEPNEYLDL NRIATTPSKE   1500
EIDNFCKQIG CYALDSKEYE RLKNSLENPS KKFIEENAAL LDLVLVDKTE   1550
YQAMKDNASN KKSLIPSTKA LDFVTMPAPQ LASAEKSSLQ KRTLSDIENE   1600
LKALGYVAIR KENLPNLEKP IVDNASKNDV LNLCSKFSLV PLSTEEYDNM   1650
RKEHTKILNI LGDPSIDFLK EKCEKYQMLI ISKHDYEEKQ EAIENPGYEF   1700
ILEKASALGY ELVSEVELDR MKQMIDSPDI DYMQEKAARN EMVLLRNEEK   1750
EALQKKIEYP SLTFLIEKAA GMNKILVDQI EYDETIRKCN HPTRMELEES   1800
CHHLNLVLLD QNEYSTLREP LENRNVEDLI NTLSKLNYIA IPNTIYQDLI   1850
GKYENPNFDY LKDSLNKMDY VAISRQDYEL MVAKYEKPQL DYLKISSEKI   1900
DHIVVPLSEY NLMVTNYRNP SLSYLKEKAV LNNHILIKED DYKNILAVSE   1950
HPTVIHLSEK ASLLNKVLVD KDDFATMSRS IEKPTIDFLS TKALSMGKIL   2000
VNESTHKRNE KLLSEPDSEF LTMKAKEQGL IIISEKEYSE LRDQIDRPSL   2050
DVLKEKAAIF DSIIVENIEY QQLVNTTSPC PPITYEDLKV YAHQFGMELC   2100
LQKPNKLSGA ERAERIDEQS INTTSSNSTT TSSMFTDALD DNIEELNRVE   2150
LQNNEDYTDI ISKSSTVKDA TIFIPAYENI KNSAEKLGYK LVPFEKSNIN   2200
LKNIEAPLFS KDNDDTSVAS SIDLDHLSRK AEKYGMTLIS DQEFEEYHIL   2250
KDNAVNLNGG MEEMNNPLSE NQNLAAKTTN TAQEGAFQNT VPHNDMDNEE   2300
VEYGPDDPTF TVRQLKKPAG DRNLILTSRE KTLLSRDDNI MSQNEAVYGD   2350
DISDSFVDES QEIKNDVDII KTQAMKYGML CIPESNFVGA SYASAQDMSD   2400
IVVLSASYYH NLMSPEDMKW NCVSNEELQA EVKKRGLQIA LTTKEDKKGQ   2450
ATASKHEYVS HKLNNKTSTV STKSGAKKGL AEAAATTAYE DSESHPQIEE   2500
QSHRTNHHKH HKRQQSLNSN STSKTTHSSR NTPASRRDIV ASFMSRAGSA   2550
SRTASLQTLA SLNEPSIIPA LTQTVIGEYL FKYYPRLGPF GFESRHERFF   2600
WVHPYTLTLY WSASNPILEN PANTKTKGVA ILGVESVTDP NPYPTGLYHK   2650
SIVVTTETRT IKFTCPTRQR HNIWYNSLRY LLQRNMQGIS LEDIADDPTD   2700
NMYSGKIFPL PGENTKSSSK RLSASRRSVS TRSLRHRVPQ SRSFGNLR     2748