SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNUG1
Systematic nameYER006W
DescriptionNuclear GTP-binding protein NUG1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP40010
SGD YER006W
Gene Ontology (GO)
Cellular componentGO:0005730nucleolusIDA:SGD.
Molecular functionGO:0003924GTPase activityTAS:SGD.
Biological processGO:0006364rRNA processingIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length520
Molecular weight57709

	---------+----------+----------+----------+----------+
MRVRKRQSRR TSTKLKEGIK KKASAHRKKE KKMAKKDVTW RSRSKKDPGI   50
PSNFPYKAKI LEEIEAKKMK DLEERELAKQ QRLEARKAAK EQGVDAMDED   100
MIEDDENGLA ALVESAQQAA AEYEGTPSND ADVRDDELDV IDYNIDFYGE   150
DVEGESELEK SRKAYDKIFK SVIDASDVIL YVLDARDPES TRSRKVEEAV   200
LQSQGKRLIL ILNKVDLIPP HVLEQWLNYL KSSFPTIPLR ASSGAVNGTS   250
FNRKLSQTTT ASALLESLKT YSNNSNLKRS IVVGVIGYPN VGKSSVINAL   300
LARRGGQSKA CPVGNEAGVT TSLREIKIDN KLKILDSPGI CFPSENKKRS   350
KVEHEAELAL LNALPAKHIV DPYPAVLMLV KRLAKSDEMT ESFKKLYEIP   400
PIPANDADTF TKHFLIHVAR KRGRLGKGGI PNLASAGLSV LNDWRDGKIL   450
GWVLPNTSAA ASQQDKQNLS TINTGTKQAP IAANESTIVS EWSKEFDLDG   500
LFSSLDKAID ASKDQDTMME                                     520