SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNTH2
Systematic nameYBR001C
DescriptionProbable trehalase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP35172
SGD YBR001C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0004555alpha,alpha-trehalase activityTAS:SGD.
Biological processGO:0006950response to stressTAS:SGD.
Biological processGO:0005993trehalose catabolic processTAS:SGD.
EC number 3.2.1.28

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
88Phosphothreonine.
90Phosphoserine.
112Phosphoserine.

Sequence Information
Sequence length780
Molecular weight89679

	---------+----------+----------+----------+----------+
MVDFLPKVTE INPPSEGNDG EDNIKPLSSG SEQRPLKEEG QQGGRRHHRR   50
LSSMHEYFDP FSNAEVYYGP ITDPRKQSKI HRLNRTRTMS VFNKVSDFKN   100
GMKDYTLKRR GSEDDSFLSS QGNRRFYIDN VDLALDELLA SEDTDKNHQI   150
TIEDTGPKVI KVGTANSNGF KHVNVRGTYM LSNLLQELTI AKSFGRHQIF   200
LDEARINENP VDRLSRLITT QFWTSLTRRV DLYNIAEIAR DSKIDTPGAK   250
NPRIYVPYNC PEQYEFYIQA SQMNPSLKLE VEYLPKDITA EYVKSLNDTP   300
GLLALAMEEH VNPSTGERSL VGYPYAVPGG RFNELYGWDS YLMALGLIES   350
NKVDVARGMV EHFIFEIDHY SKILNANRSY YLCRSQPPFL TDMALLVFEK   400
IGGKNNPNAI QLLKRAFRAA IKEYKEVWMS SPRLDSLTGL SCYHSDGIGI   450
PPETEPDHFD TILLPYAEKY NVTLEKLRYL YNEGMIKEPK LDAFFLHDRA   500
VRESGHDTTY RFEGVCAYLA TIDLNSLLYK YEKDIAFVIK EYFGNEYKDE   550
NDGTVTDSEH WEELAELRKT RINKYMWDED SGFFFYYNTK LKCRTSYESA   600
TTFWSLWAGL ATEEQAKITV EKALPQLEML GGLVACTEKS RGPISIDRPI   650
RQWDYPFGWA PHQILAWKGL SAYGYQQVAT RLAYRWLYMI TKSFVDYNGM   700
VVEKYDVTRG TDPHRVDAEY GNQGADFKGV ATEGFGWVNT SYLLGLKYMN   750
NHARRALAAC SPPLPFFNSL KPSEKKLYYL                          780