SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNSP1
Systematic nameYJL041W
DescriptionNucleoporin NSP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP14907
SGD YJL041W
Gene Ontology (GO)
Cellular componentGO:0005643nuclear poreIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0005198structural molecule activityTAS:SGD.
Biological processGO:0006406mRNA export from nucleusTAS:SGD.
Biological processGO:0006609mRNA-binding (hnRNP) protein import into nu...TAS:SGD.
Biological processGO:0006607NLS-bearing substrate import into nucleusTAS:SGD.
Biological processGO:0006999nuclear pore organization and biogenesisIGI:SGD.
Biological processGO:0000055ribosomal large subunit export from nucleusIMP:SGD.
Biological processGO:0006610ribosomal protein import into nucleusTAS:SGD.
Biological processGO:0006407rRNA export from nucleusTAS:SGD.
Biological processGO:0006408snRNA export from nucleusTAS:SGD.
Biological processGO:0006608snRNP protein import into nucleusTAS:SGD.
Biological processGO:0006409tRNA export from nucleusTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
456Phosphoserine.

Sequence Information
Sequence length823
Molecular weight86516

	---------+----------+----------+----------+----------+
MNFNTPQQNK TPFSFGTANN NSNTTNQNSS TGAGAFGTGQ STFGFNNSAP   50
NNTNNANSSI TPAFGSNNTG NTAFGNSNPT SNVFGSNNST TNTFGSNSAG   100
TSLFGSSSAQ QTKSNGTAGG NTFGSSSLFN NSTNSNTTKP AFGGLNFGGG   150
NNTTPSSTGN ANTSNNLFGA TANANKPAFS FGATTNDDKK TEPDKPAFSF   200
NSSVGNKTDA QAPTTGFSFG SQLGGNKTVN EAAKPSLSFG SGSAGANPAG   250
ASQPEPTTNE PAKPALSFGT ATSDNKTTNT TPSFSFGAKS DENKAGATSK   300
PAFSFGAKPE EKKDDNSSKP AFSFGAKSNE DKQDGTAKPA FSFGAKPAEK   350
NNNETSKPAF SFGAKSDEKK DGDASKPAFS FGAKPDENKA SATSKPAFSF   400
GAKPEEKKDD NSSKPAFSFG AKSNEDKQDG TAKPAFSFGA KPAEKNNNET   450
SKPAFSFGAK SDEKKDGDAS KPAFSFGAKS DEKKDSDSSK PAFSFGTKSN   500
EKKDSGSSKP AFSFGAKPDE KKNDEVSKPA FSFGAKANEK KESDESKSAF   550
SFGSKPTGKE EGDGAKAAIS FGAKPEEQKS SDTSKPAFTF GAQKDNEKKT   600
EESSTGKSTA DVKSSDSLKL NSKPVELKPV SLDNKTLDDL VTKWTNQLTE   650
SASHFEQYTK KINSWDQVLV KGGEQISQLY SDAVMAEHSQ NKIDQSLQYI   700
ERQQDELENF LDNFETKTEA LLSDVVSTSS GAAANNNDQK RQQAYKTAQT   750
LDENLNSLSS NLSSLIVEIN NVSNTFNKTT NIDINNEDEN IQLIKILNSH   800
FDALRSLDDN STSLEKQINS IKK                                823