SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNPA3
Systematic nameYJR072C
AliasEPA1
DescriptionGTPase NPA3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP47122
SGD YJR072C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0016887ATPase activityIDA:SGD.
EC number 3.6.-.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
304Phosphoserine.
308Phosphoserine.
313Phosphoserine.
352Phosphoserine.

Sequence Information
Sequence length385
Molecular weight43195

	---------+----------+----------+----------+----------+
MSLSTIICIG MAGSGKTTFM QRLNSHLRAE KTPPYVINLD PAVLRVPYGA   50
NIDIRDSIKY KKVMENYQLG PNGAIVTSLN LFSTKIDQVI RLVEQKKDKF   100
QNCIIDTPGQ IECFVWSASG AIITESFASS FPTVIAYIVD TPRNSSPTTF   150
MSNMLYACSI LYKTKLPMIV VFNKTDVCKA DFAKEWMTDF ESFQAAIKED   200
QDLNGDNGLG SGYMSSLVNS MSLMLEEFYS QLDVVGVSSF TGDGFDEFMQ   250
CVDKKVDEYD QYYKQEREKA LNLKKKKEEM RKQKSLNGLM KDLGLNEKSS   300
AAASDNDSID AISDLEEDAN DGLVDRDEDE GVEREYTFPG EERTKGEVNE   350
NSAPDLQRRY QEAMQQVGKT ASSETAENIA KYIRN                   385