SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNOT4
Systematic nameYER068W
AliasCCL1, MOT2, SIG1, SSF1
DescriptionGeneral negative regulator of transcription subunit 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP34909
SGD YER068W
Gene Ontology (GO)
Cellular componentGO:0030015CCR4-NOT core complexIPI:SGD.
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0016564transcriptional repressor activityTAS:SGD.
Molecular functionGO:0004842ubiquitin-protein ligase activityIDA:SGD.
Biological processGO:0000747conjugation with cellular fusionIMP:SGD.
Biological processGO:0000289poly(A) tail shorteningIDA:SGD.
Biological processGO:0016567protein ubiquitinationIDA:SGD.
Biological processGO:0006357regulation of transcription from RNA polyme...IPI:SGD.
Biological processGO:0006368RNA elongation from RNA polymerase II promoterIMP:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
298Phosphoserine.
310Phosphothreonine.
312Phosphoserine.

Sequence Information
Sequence length587
Molecular weight65354

	---------+----------+----------+----------+----------+
MMNPHVQENL QAIHNALSNF DTSFLSEDEE DYCPLCIEPM DITDKNFFPC   50
PCGYQICQFC YNNIRQNPEL NGRCPACRRK YDDENVRYVT LSPEELKMER   100
AKLARKEKER KHREKERKEN EYTNRKHLSG TRVIQKNLVY VVGINPPVPY   150
EEVAPTLKSE KYFGQYGKIN KIVVNRKTPH SNNTTSEHYH HHSPGYGVYI   200
TFGSKDDAAR CIAQVDGTYM DGRLIKAAYG TTKYCSSYLR GLPCPNPNCM   250
FLHEPGEEAD SFNKRELHNK QQAQQQSGGT AFTRSGIHNN ISTSTAGSNT   300
NLLSENFTGT PSPAAMRAQL HHDSHTNAGT PVLTPAPVPA GSNPWGVTQS   350
ATPVTSINLS KNSSSINLPT LNDSLGHHTT PTTENTITST TTTTNTNATS   400
HSHGSKKKQS LAAEEYKDPY DALGNAVDFL DARLHSLSNY QKRPISIKSN   450
IIDEETYKKY PSLFSWDKIE ASKKSDNTLA NKLVEILAIK PIDYTASVVQ   500
FLQSVNVGVN DNITITDNTK TPTQPIRLQT VSQQIQPPLN VSTPPPGIFG   550
PQHKVPIQQQ QMGDTSSRNS SDLLNQLING RKIIAGN                 587