SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNOT1
Systematic nameYCR093W
AliasCDC39, ROS1
DescriptionGeneral negative regulator of transcription subunit 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP25655
SGD YCR093W
Gene Ontology (GO)
Cellular componentGO:0030015CCR4-NOT core complexIPI:SGD.
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:00001753'-5'-exoribonuclease activityIDA:SGD.
Biological processGO:0000122negative regulation of transcription from R...TAS:SGD.
Biological processGO:0000289poly(A) tail shorteningIDA:SGD.
Biological processGO:0007124pseudohyphal growthIMP:SGD.
Biological processGO:0000074regulation of progression through cell cycleIMP:SGD.
Biological processGO:0000749response to pheromone during conjugation wi...IMP:SGD.
Biological processGO:0006368RNA elongation from RNA polymerase II promoterIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2102Phosphothreonine.

Sequence Information
Sequence length2108
Molecular weight240335

	---------+----------+----------+----------+----------+
MLSATYRDLN TASNLETSKE KQAAQIVIAQ ISLLFTTLNN DNFESVEREI   50
RHILDRSSVD IYIKVWERLL TLSSRDILQA GKFLLQENLL HRLLLEFAKD   100
LPKKSTDLIE LLKERTFNNQ EFQKQTGITL SLFIDLFDKS ANKDIIESLD   150
RSSQINDFKT IKMNHTNYLR NFFLQTTPET LESNLRDLLH SLEGESLNDL   200
LALLLSEILS PGSQNLQNDP TRSWLTPPMV LDATNRGNVI ARSISSLQAN   250
QINWNRVFNL MSTKYFLSAP LMPTTASLSC LFAALHDGPV IDEFFSCDWK   300
VIFKLDLAIQ LHKWSVQNGC FDLLNAEGTR KVSETIPNTK QSLLYLLSIA   350
SLNLELFLQR EELSDGPMLA YFQECFFEDF NYAPEYLILA LVKEMKRFVL   400
LIENRTVIDE ILITLLIQVH NKSPSSFKDV ISTITDDSKI VDAAKIIINS   450
DDAPIANFLK SLLDTGRLDT VINKLPFNEA FKILPCARQI GWEGFDTFLK   500
TKVSPSNVDV VLESLEVQTK MTDTNAPFRS LKTFDLFAFH SLIEVLNKCP   550
LDVLQLQRFE SLEFSLLIRF PRLINFGFGH DEAILANGDI AGINNDIEKE   600
MQNYLQKMYS GELAIKDVIE LLRRLRDSDL PRDQEVFTCI THAVIAESTF   650
FQDYPLDALA TTSVLFGSMI LFQLLRGFVL DVAFRIIMRF AKEPPESKMF   700
KFAVQAIYAF RIRLAEYPQY CKDLLRDVPA LKSQAQVYQS IVEAATLANA   750
PKERSRPVQE MIPLKFFAVD EVSCQINQEG APKDVVEKVL FVLNNVTLAN   800
LNNKVDELKK SLTPNYFSWF STYLVTQRAK TEPNYHDLYS KVIVAMGSGL   850
LHQFMVNVTL RQLFVLLSTK DEQAIDKKHL KNLASWLGCI TLALNKPIKH   900
KNIAFREMLI EAYKENRLEI VVPFVTKILQ RASESKIFKP PNPWTVGILK   950
LLIELNEKAN WKLSLTFEVE VLLKSFNLTT KSLKPSNFIN TPEVIETLSG   1000
ALGSITLEQQ QTEQQRQIIL MQQHQQQMLI YQQRQQQQQQ RQQQQQHHIS   1050
ANTIADQQAA FGGEGSISHD NPFNNLLGST IFVTHPDLKR VFQMALAKSV   1100
REILLEVVEK SSGIAVVTTT KIILKDFATE VDESKLKTAA IIMVRHLAQS   1150
LARATSIEPL KEGIRSTMQS LAPNLMSLSS SPAEELDTAI NENIGIALVL   1200
IEKASMDKST QDLADQLMQA IAIRRYHKER RADQPFITQN TNPYSLSLPE   1250
PLGLKNTGVT PQQFRVYEEF GKNIPNLDVI PFAGLPAHAP PMTQNVGLTQ   1300
PQQQQAQMPT QILTSEQIRA QQQQQQLQKS RLNQPSQSAQ PPGVNVPNPQ   1350
GGIAAVQSDL EQNQRVLVHL MDILVSQIKE NATKNNLAEL GDQNQIKTII   1400
FQILTFIAKS AQKDQLALKV SQAVVNSLFA TSESPLCREV LSLLLEKLCS   1450
LSLVARKDVV WWLVYALDSR KFNVPVIRSL LEVNLIDATE LDNVLVTAMK   1500
NKMENSTEFA MKLIQNTVLS DDPILMRMDF IKTLEHLASS EDENVKKFIK   1550
EFEDTKIMPV RKGTKTTRTE KLYLVFTEWV KLLQRVENND VITTVFIKQL   1600
VEKGVISDTD NLLTFVKSSL ELSVSSFKES DPTDEVFIAI DALGSLIIKL   1650
LILQGFKDDT RRDYINAIFS VIVLVFAKDH SQEGTTFNER PYFRLFSNIL   1700
YEWATIRTHN FVRISDSSTR QELIEFDSVF YNTFSGYLHA LQPFAFPGFS   1750
FAWVTLLSHR MLLPIMLRLP NKIGWEKLML LIIDLFKFLD QYTSKHAVSD   1800
AVSVVYKGTL RVILGISNDM PSFLIENHYE LMNNLPPTYF QLKNVILSAI   1850
PKNMTVPNPY DVDLNMEDIP ACKELPEVFF DPVIDLHSLK KPVDNYLRIP   1900
SNSLLRTILS AIYKDTYDIK KGVGYDFLSV DSKLIRAIVL HVGIEAGIEY   1950
KRTSSNAVFN TKSSYYTLLF NLIQNGSIEM KYQIILSIVE QLRYPNIHTY   2000
WFSFVLMNMF KSDEWNDQKL EVQEIILRNF LKRIIVNKPH TWGVSVFFTQ   2050
LINNNDINLL DLPFVQSVPE IKLILQQLVK YSKKYTTSEQ DDQSATINRR   2100
QTPLQSNA                                                 2108