SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNOP4
Systematic nameYPL043W
AliasNOP77
DescriptionNucleolar protein 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP37838
SGD YPL043W
Gene Ontology (GO)
Cellular componentGO:0005730nucleolusTAS:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006364rRNA processingTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
247Phosphoserine.
379Phosphothreonine.

Sequence Information
Sequence length685
Molecular weight77825

	---------+----------+----------+----------+----------+
MEETIENVEV PSSNVSKQND DGLDMKTLFV RSIPQDVTDE QLADFFSNFA   50
PIKHAVVVKD TNKRSRGFGF VSFAVEDDTK EALAKARKTK FNGHILRVDI   100
AKRRDRSKKT SEVVEKSTPE SSEKITGQNN EDEDDADGED SMLKGKPKLI   150
IRNMPWSCRD PVKLKKIFGR YGTVVEATIP RKRDGKLCGF AFVTMKKISN   200
CRIALENTKD LKIDGRKVAV DFAVQKNRWE DYKKAQPEMN DKDDNESGNE   250
DAEENHDDEE DENEEEDRQV DQASKNKESK RKAQNKREDF SVFVRNVPYD   300
ATEESLAPHF SKFGSVKYAL PVIDKSTGLA KGTAFVAFKD QYTYNECIKN   350
APAAGSTSLL IGDDVMPEYV YEGRVLSITP TLVREDAGRM AEKNAAKRKE   400
ALGKAPGEKD RRNLYLLNEG RVVEGSKMAD LLTNTDMEIR EKSYKLRVEQ   450
LKKNPSLHLS MTRLAIRNLP RAMNDKALKA LARKAVVEFA TEVKNKERHP   500
LSKEEIIRST KEKYKFMGPD EIEAQKKKDK KSGVVKQAKV IMEVKGSTAG   550
RSRGYGFVEF RDHKNALMGL RWLNCHAVTS DEILEGLNDD EKKQVDNDLG   600
KGRRLCVEFA IENSNVVKRR REQLKQARTK RTRPDNEDTG DVGESENKKP   650
KKEEATTPTN PDDKKMGDDI KRIIGFKRKR KHAKK                   685