SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNOG2
Systematic nameYNR053C
DescriptionNucleolar GTP-binding protein 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53742
SGD YNR053C
Gene Ontology (GO)
Cellular componentGO:0005730nucleolusIDA:SGD.
Cellular componentGO:0005654nucleoplasmIDA:SGD.
Molecular functionGO:0003924GTPase activityTAS:SGD.
Biological processGO:0000055ribosomal large subunit export from nucleusIMP:SGD.
Biological processGO:0042255ribosome assemblyTAS:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length486
Molecular weight55489

	---------+----------+----------+----------+----------+
MGTGKKEKSR RIREGDTKDG NLRVKGENFY RDSKRVKFLN MYTSGKEIRN   50
KKGNLIRAAS FQDSTIPDAR VQPDRRWFGN TRVISQDALQ HFRSALGETQ   100
KDTYQVLLRR NKLPMSLLEE KDADESPKAR ILDTESYADA FGPKAQRKRP   150
RLAASNLEDL VKATNEDITK YEEKQVLDAT LGLMGNQEDK ENGWTSAAKE   200
AIFSKGQSKR IWNELYKVID SSDVVIHVLD ARDPLGTRCK SVEEYMKKET   250
PHKHLIYVLN KCDLVPTWVA AAWVKHLSKE RPTLAFHASI TNSFGKGSLI   300
QLLRQFSQLH TDRKQISVGF IGYPNTGKSS IINTLRKKKV CQVAPIPGET   350
KVWQYITLMK RIFLIDCPGI VPPSSKDSEE DILFRGVVRV EHVTHPEQYI   400
PGVLKRCQVK HLERTYEISG WKDATEFIEI LARKQGRLLK GGEPDESGVS   450
KQILNDFNRG KIPWFVLPPE KEGEEKPKKK EVEKTA                  486