SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNIP1
Systematic nameYMR309C
DescriptionEukaryotic translation initiation factor 3 93 kDa subunit

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionP32497
SGD YMR309C
Gene Ontology (GO)
Cellular componentGO:0005852eukaryotic translation initiation factor 3 ...IDA:SGD.
Cellular componentGO:0043614multi-eIF complexIDA:SGD.
Molecular functionGO:0003743translation initiation factor activityIDA:SGD.
Biological processGO:0006413translational initiationIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
98Phosphoserine.
99Phosphoserine.
103Phosphoserine.
809Phosphoserine.

Sequence Information
Sequence length812
Molecular weight93204

	---------+----------+----------+----------+----------+
MSRFFSSNYE YDVASSSSEE DLLSSSEEDL LSSSSSESEL DQESDDSFFN   50
ESESESEADV DSDDSDAKPY GPDWFKKSEF RKQGGGSNKF LKSSNYDSSD   100
EESDEEDGKK VVKSAKEKLL DEMQDVYNKI SQAENSDDWL TISNEFDLIS   150
RLLVRAQQQN WGTPNIFIKV VAQVEDAVNN TQQADLKNKA VARAYNTTKQ   200
RVKKVSRENE DSMAKFRNDP ESFDKEPTAD LDISANGFTI SSSQGNDQAV   250
QEDFFTRLQT IIDSRGKKTV NQQSLISTLE ELLTVAEKPY EFIMAYLTLI   300
PSRFDASANL SYQPIDQWKS SFNDISKLLS ILDQTIDTYQ VNEFADPIDF   350
IEDEPKEDSD GVKRILGSIF SFVERLDDEF MKSLLNIDPH SSDYLIRLRD   400
EQSIYNLILR TQLYFEATLK DEHDLERALT RPFVKRLDHI YYKSENLIKI   450
METAAWNIIP AQFKSKFTSK DQLDSADYVD NLIDGLSTIL SKQNNIAVQK   500
RAILYNIYYT ALNKDFQTAK DMLLTSQVQT NINQFDSSLQ ILFNRVVVQL   550
GLSAFKLCLI EECHQILNDL LSSSHLREIL GQQSLHRISL NSSNNASADE   600
RARQCLPYHQ HINLDLIDVV FLTCSLLIEI PRMTAFYSGI KVKRIPYSPK   650
SIRRSLEHYD KLSFQGPPET LRDYVLFAAK SMQKGNWRDS VKYLREIKSW   700
ALLPNMETVL NSLTERVQVE SLKTYFFSFK RFYSSFSVAK LAELFDLPEN   750
KVVEVLQSVI AELEIPAKLN DEKTIFVVEK GDEITKLEEA MVKLNKEYKI   800
AKERLNPPSN RR                                            812