SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNGR1
Systematic nameYBR212W
AliasRBP1
DescriptionNegative growth regulatory protein NGR1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP32831
SGD YBR212W
Gene Ontology (GO)
Cellular componentGO:0000932cytoplasmic mRNA processing bodyIDA:SGD.
Cellular componentGO:0048471perinuclear region of cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0003723RNA bindingIDA:SGD.
Molecular functionGO:0003697single-stranded DNA bindingIDA:SGD.
Molecular functionGO:0045182translation regulator activityIMP:SGD.
Biological processGO:0007005mitochondrion organization and biogenesisIMP:SGD.
Biological processGO:0040008regulation of growthIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
524Phosphoserine.

Sequence Information
Sequence length672
Molecular weight75024

	---------+----------+----------+----------+----------+
MMSNVANASQ RQENPYIIPL PPSSTVETST EPPRTLWMGD LDPSFDEATI   50
EEIWSKLDKK VIVKLIRAKK NLLIPCSSTS SSNNNTSEEN AENQQSASNS   100
TDQLDNSQMI NINGISFIDP STTQLHHAGY CFVEFETQKD AKFALSLNAT   150
PLPNFYSPTT NSQTNPTFKR TFRLNWASGA TLQSSIPSTP EFSLFVGDLS   200
PTATEADLLS LFQTRFKSVK TVRVMTDPLT GSSRCFGFVR FGDEDERRRA   250
LIEMSGKWFQ GRALRVAYAT PRNNMMLQLQ EQQQQQQQLQ QQHQQLDQED   300
NNGPLLIKTA NNLIQNNSNM LPLNALHNAP PMHLNEGGIS NMRVNDSLPS   350
NTYNTDPTNT TVFVGGLVPK TTEFQLRSLF KPFGPILNVR IPNGKNCGFV   400
KFEKRIDAEA SIQGLQGFIV GGSPIRLSWG RPSSSNAKTN STIMGASQYM   450
SSNGLRAPSA ASSVDNSKQI LEQYAEDKRR LFLHQQQQQQ QQQQQDGNFS   500
MEQMAHNNYY NYNNYDYHRN KNGSHSDLVN LQRSNVPYMQ EDGALYPHQY   550
SSPSYSLHPT GNQFSNATNN LPQFGNAMSI SMQLPNGNSN KTASSMNTNP   600
NTNMIMNSNM NMNMNVNPVP YGMGNGANMY DVSRMMTPPL NIAPNSNNSK   650
SSIMNKHPNR NNVPPIHPSL LH                                 672