SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNEW1
Systematic nameYPL226W
Description[NU+] prion formation protein 1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ08972
SGD YPL226W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
110Phosphothreonine.
125Phosphoserine.
1191Phosphothreonine.

Sequence Information
Sequence length1196
Molecular weight134331

	---------+----------+----------+----------+----------+
MPPKKFKDLN SFLDDQPKDP NLVASPFGGY FKNPAADAGS NNASKKSSYQ   50
QQRNWKQGGN YQQGGYQSYN SNYNNYNNYN NYNNYNNYNN YNKYNGQGYQ   100
KSTYKQSAVT PNQSGTPTPS ASTTSLTSLN EKLSNLELTP ISQFLSKIPE   150
CQSITDCKNQ IKLIIEEFGK EGNSTGEKIE EWKIVDVLSK FIKPKNPSLV   200
RESAMLIISN IAQFFSGKPP QEAYLLPFFN VALDCISDKE NTVKRAAQHA   250
IDSLLNCFPM EALTCFVLPT ILDYLSSGAK WQAKMAALSV VDRIREDSAN   300
DLLELTFKDA VPVLTDVATD FKPELAKQGY KTLLDYVSIL DNLDLSPRYK   350
LIVDTLQDPS KVPESVKSLS SVTFVAEVTE PSLSLLVPIL NRSLNLSSSS   400
QEQLRQTVIV VENLTRLVNN RNEIESFIPL LLPGIQKVVD TASLPEVREL   450
AEKALNVLKE DDEADKENKF SGRLTLEEGR DFLLDHLKDI KADDSCFVKP   500
YMNDETVIKY MSKILTVDSN VNDWKRLEDF LTAVFGGSDS QREFVKQDFI   550
HNLRALFYQE KERADEDEGI EIVNTDFSLA YGSRMLLNKT NLRLLKGHRY   600
GLCGRNGAGK STLMRAIANG QLDGFPDKDT LRTCFVEHKL QGEEGDLDLV   650
SFIALDEELQ STSREEIAAA LESVGFDEER RAQTVGSLSG GWKMKLELAR   700
AMLQKADILL LDEPTNHLDV SNVKWLEEYL LEHTDITSLI VSHDSGFLDT   750
VCTDIIHYEN KKLAYYKGNL AAFVEQKPEA KSYYTLTDSN AQMRFPPPGI   800
LTGVKSNTRA VAKMTDVTFS YPGAQKPSLS HVSCSLSLSS RVACLGPNGA   850
GKSTLIKLLT GELVPNEGKV EKHPNLRIGY IAQHALQHVN EHKEKTANQY   900
LQWRYQFGDD REVLLKESRK ISEDEKEMMT KEIDIDDGRG KRAIEAIVGR   950
QKLKKSFQYE VKWKYWKPKY NSWVPKDVLV EHGFEKLVQK FDDHEASREG   1000
LGYRELIPSV ITKHFEDVGL DSEIANHTPL GSLSGGQLVK VVIAGAMWNN   1050
PHLLVLDEPT NYLDRDSLGA LAVAIRDWSG GVVMISHNNE FVGALCPEQW   1100
IVENGKMVQK GSAQVDQSKF EDGGNADAVG LKASNLAKPS VDDDDSPANI   1150
KVKQRKKRLT RNEKKLQAER RRLRYIEWLS SPKGTPKPVD TDDEED       1196