SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNEO1
Systematic nameYIL048W
DescriptionProbable phospholipid-transporting ATPase NEO1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP40527
SGD YIL048W
Gene Ontology (GO)
Cellular componentGO:0005768endosomeIDA:SGD.
Cellular componentGO:0000139Golgi membraneIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0006897endocytosisIMP:SGD.
Biological processGO:0006890retrograde vesicle-mediated transport, Golg...IMP:SGD.
Biological processGO:0007033vacuole organization and biogenesisIMP:SGD.
EC number 3.6.3.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
102Phosphoserine.
551Phosphoserine.

Sequence Information
Sequence length1151
Molecular weight130218

	---------+----------+----------+----------+----------+
MPNPPSFKSH KQNLFNSNNN QHANSVDSFD LHLDDSFDAA LDSLQINNNP   50
EPLSKHNTVG DRESFEMRTV DDLDNFSNHS SDSHRKSSNT DTHPLMYDNR   100
LSQDDNFKFT NIASSPPSSS NNIFSKALSY LKVSNTKNWS KFGSPIELSD   150
QHIEREIHPD TTPVYDRNRY VSNELSNAKY NAVTFVPTLL YEQFKFFYNL   200
YFLVVALSQA VPALRIGYLS SYIVPLAFVL TVTMAKEAID DIQRRRRDRE   250
SNNELYHVIT RNRSIPSKDL KVGDLIKVHK GDRIPADLVL LQSSEPSGES   300
FIKTDQLDGE TDWKLRVACP LTQNLSENDL INRISITASA PEKSIHKFLG   350
KVTYKDSTSN PLSVDNTLWA NTVLASSGFC IACVVYTGRD TRQAMNTTTA   400
KVKTGLLELE INSISKILCA CVFALSILLV AFAGFHNDDW YIDILRYLIL   450
FSTIIPVSLR VNLDLAKSVY AHQIEHDKTI PETIVRTSTI PEDLGRIEYL   500
LSDKTGTLTQ NDMQLKKIHL GTVSYTSETL DIVSDYVQSL VSSKNDSLNN   550
SKVALSTTRK DMSFRVRDMI LTLAICHNVT PTFEDDELTY QAASPDEIAI   600
VKFTESVGLS LFKRDRHSIS LLHEHSGKTL NYEILQVFPF NSDSKRMGII   650
VRDEQLDEYW FMQKGADTVM SKIVESNDWL EEETGNMARE GLRTLVIGRK   700
KLNKKIYEQF QKEYNDASLS MLNRDQQMSQ VITKYLEHDL ELLGLTGVED   750
KLQKDVKSSI ELLRNAGIKI WMLTGDKVET ARCVSISAKL ISRGQYVHTI   800
TKVTRPEGAF NQLEYLKINR NACLLIDGES LGMFLKHYEQ EFFDVVVHLP   850
TVIACRCTPQ QKADVALVIR KMTGKRVCCI GDGGNDVSMI QCADVGVGIV   900
GKEGKQASLA ADFSITQFCH LTELLLWHGR NSYKRSAKLA QFVMHRGLII   950
AICQAVYSIC SLFEPIALYQ GWLMVGYATC YTMAPVFSLT LDHDIEESLT   1000
KIYPELYKEL TEGKSLSYKT FFVWVLLSLF QGSVIQLFSQ AFTSLLDTDF   1050
TRMVAISFTA LVVNELIMVA LEIYTWNKTM LVTEIATLLF YIVSVPFLGD   1100
YFDLGYMTTV NYYAGLLVIL LISIFPVWTA KAIYRRLHPP SYAKVQEFAT   1150
P                                                        1151