SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNDE1
Systematic nameYMR145C
AliasNDH1
DescriptionExternal NADH-ubiquinone oxidoreductase 1, mitochondrial

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP40215
SGD YMR145C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0003954NADH dehydrogenase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0001300chronological cell agingIMP:SGD.
Biological processGO:0019655glucose catabolic process to ethanolIMP:SGD.
Biological processGO:0006116NADH oxidationIDA:SGD.
EC number 1.6.5.3

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length560
Molecular weight62774

	---------+----------+----------+----------+----------+
MIRQSLMKTV WANSSRFSLQ SKSGLVKYAK NRSFHAARNL LEDKKVILQK   50
VAPTTGVVAK QSFFKRTGKF TLKALLYSAL AGTAYVSYSL YREANPSTQV   100
PQSDTFPNGS KRKTLVILGS GWGSVSLLKN LDTTLYNVVV VSPRNYFLFT   150
PLLPSTPVGT IELKSIVEPV RTIARRSHGE VHYYEAEAYD VDPENKTIKV   200
KSSAKNNDYD LDLKYDYLVV GVGAQPNTFG TPGVYEYSSF LKEISDAQEI   250
RLKIMSSIEK AASLSPKDPE RARLLSFVVV GGGPTGVEFA AELRDYVDQD   300
LRKWMPELSK EIKVTLVEAL PNILNMFDKY LVDYAQDLFK EEKIDLRLKT   350
MVKKVDATTI TAKTGDGDIE NIPYGVLVWA TGNAPREVSK NLMTKLEEQD   400
SRRGLLIDNK LQLLGAKGSI FAIGDCTFHP GLFPTAQVAH QEGEYLAQYF   450
KKAYKIDQLN WKMTHAKDDS EVARLKNQIV KTQSQIEDFK YNHKGALAYI   500
GSDKAIADLA VGEAKYRLAG SFTFLFWKSA YLAMCLSFRN RVLVAMDWAK   550
VYFLGRDSSI                                                560