SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameNAM7
Systematic nameYMR080C
AliasIFS2, MOF4, UPF1
DescriptionATP-dependent helicase NAM7

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP30771
SGD YMR080C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005844polysomeTAS:SGD.
Molecular functionGO:0004004ATP-dependent RNA helicase activityTAS:SGD.
Molecular functionGO:0003676nucleic acid bindingTAS:SGD.
Biological processGO:0030466chromatin silencing at silent mating-type c...IGI:SGD.
Biological processGO:0000184mRNA catabolic process, nonsense-mediated d...TAS:SGD.
Biological processGO:0006449regulation of translational terminationTAS:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number 3.6.1.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
869Phosphoserine.

Sequence Information
Sequence length971
Molecular weight109430

	---------+----------+----------+----------+----------+
MVGSGSHTPY DISNSPSDVN VQPATQLNST LVEDDDVDNQ LFEEAQVTET   50
GFRSPSASDN SCAYCGIDSA KCVIKCNSCK KWFCNTKNGT SSSHIVNHLV   100
LSHHNVVSLH PDSDLGDTVL ECYNCGRKNV FLLGFVSAKS EAVVVLLCRI   150
PCAQTKNANW DTDQWQPLIE DRQLLSWVAE QPTEEEKLKA RLITPSQISK   200
LEAKWRSNKD ATINDIDAPE EQEAIPPLLL RYQDAYEYQR SYGPLIKLEA   250
DYDKQLKESQ ALEHISVSWS LALNNRHLAS FTLSTFESNE LKVAIGDEMI   300
LWYSGMQHPD WEGRGYIVRL PNSFQDTFTL ELKPSKTPPP THLTTGFTAE   350
FIWKGTSYDR MQDALKKFAI DKKSISGYLY YKILGHQVVD ISFDVPLPKE   400
FSIPNFAQLN SSQSNAVSHV LQRPLSLIQG PPGTGKTVTS ATIVYHLSKI   450
HKDRILVCAP SNVAVDHLAA KLRDLGLKVV RLTAKSREDV ESSVSNLALH   500
NLVGRGAKGE LKNLLKLKDE VGELSASDTK RFVKLVRKTE AEILNKADVV   550
CCTCVGAGDK RLDTKFRTVL IDESTQASEP ECLIPIVKGA KQVILVGDHQ   600
QLGPVILERK AADAGLKQSL FERLISLGHV PIRLEVQYRM NPYLSEFPSN   650
MFYEGSLQNG VTIEQRTVPN SKFPWPIRGI PMMFWANYGR EEISANGTSF   700
LNRIEAMNCE RIITKLFRDG VKPEQIGVIT PYEGQRAYIL QYMQMNGSLD   750
KDLYIKVEVA SVDAFQGREK DYIILSCVRA NEQQAIGFLR DPRRLNVGLT   800
RAKYGLVILG NPRSLARNTL WNHLLIHFRE KGCLVEGTLD NLQLCTVQLV   850
RPQPRKTERP MNAQFNVESE MGDFPKFQDF DAQSMVSFSG QIGDFGNAFV   900
DNTELSSYIN NEYWNFENFK SAFSQKQNRN EIDDRNLYQE EASHLNSNFA   950
RELQREEQKH ELSKDFSNLG I                                  971