SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMYO4
Systematic nameYAL029C
AliasSHE1
DescriptionMyosin-4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP32492
SGD YAL029C
Gene Ontology (GO)
Cellular componentGO:0005933cellular budIDA:SGD.
Cellular componentGO:0031941filamentous actinIDA:SGD.
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0051015actin filament bindingIDA:SGD.
Molecular functionGO:0000146microfilament motor activityIDA:SGD.
Biological processGO:0048309endoplasmic reticulum inheritanceIMP:SGD.
Biological processGO:0007534gene conversion at mating-type locusIMP:SGD.
Biological processGO:0008298intracellular mRNA localizationIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1471
Molecular weight169344

	---------+----------+----------+----------+----------+
MSFEVGTKCW YPHKEQGWIG GEVTKNDFFE GTFHLELKLE DGETVSIETN   50
SFENDDDHPT LPVLRNPPIL ESTDDLTTLS YLNEPAVLHA IKKRYMNGQI   100
YTYSGIVLIA ANPFDKVDHL YSREMIQNYS SKRKDELEPH LFAIAEEAYR   150
FMVHEKANQT VVVSGESGAG KTVSAKYIMR YFASVQESNN REGEVEMSQI   200
ESQILATNPI MEAFGNAKTT RNDNSSRFGK YLQILFDENT TIRGSKIRTY   250
LLEKSRLVYQ PETERNYHIF YQILEGLPEP VKQELHLSSP KDYHYTNQGG   300
QPNIAGIDEA REYKITTDAL SLVGINHETQ LGIFKILAGL LHIGNIEMKM   350
TRNDASLSSE EQNLQIACEL LGIDPFNFAK WIVKKQIVTR SEKIVTNLNY   400
NQALIARDSV AKFIYSTLFD WLVDNINKTL YDPELDQQDH VFSFIGILDI   450
YGFEHFEKNS FEQFCINYAN EKLQQEFNQH VFKLEQEEYV KEEIEWSFIE   500
FSDNQPCIDL IENKLGILSL LDEESRLPSG SDESWASKLY SAFNKPPSNE   550
VFSKPRFGQT KFIVSHYAVD VEYEVEGFIE KNRDSVSLGH LDVFKATTNP   600
IFKQILDNRE LRSDDAPEEQ NTEKKIMIPA RLSQKKPTLG SMFKKSLGEL   650
MAIINSTNVH YIRCIKPNSE KKPWEFDNLM VLSQLRACGV LETIRISCAG   700
FPSRWTFDEF VQRYFLLTDY SLWSGILYNP DLPKEAIVNF CQSILDATIS   750
DSAKYQIGNT KIFFKAGMLA FLEKLRTNKM NEICIIIQKK IRARYYRLQY   800
LQTMESIKKC QSQIRSLLVR TRVDHELKTR AAILLQTNIR ALWKREYYRA   850
AIGQIIKLQC TCKRKLILDS VNRKFMLMAA VIIQSYIRSY GHKTDYRTLK   900
RSSILVQSAM RMQLARRRYI VLQKEVEERN IRASYGIGLL EEAIEFKNSF   950
ILNLEMLNDS YTRLTQLLQG DLSNIPSKQR QEYETIVNGY NDKISKLKTL   1000
QVEIMNTLNK KNALKERKKK QSSLIQSHMQ SLAAIKGNKP SRLSDEVKSM   1050
KQELAFIENV IAQDFTTTYS ANKNDKVKGL GIAGQQVKPK LVNVIRRESG   1100
NPDLLELLMD LNCYTLEVTE GYLKKVNVTE VNGDNVLGPI HVITTVVSSL   1150
VRNGLLIQSS KFISKVLLTV ESIVMSLPKD ETMLGGIFWL SNLSRLPAFA   1200
ANQKTLYEAN GGDEKDKLTL IYLNDLENET LKVFDKIYST WLVKFMKHAS   1250
AHIEIFDMVL NEKLFKNSGD EKFAKLFTFL NEFDAVLCKF QVVDSMHTKI   1300
FNDTLKYLNV MLFNDLITKC PALNWKYGYE VDRNIERLVS WFEPRIEDVR   1350
PNLIQIIQAV KILQLKISNL NEFKLLFDFW YALNPAQIQA ILLKYKPANK   1400
GEAGVPNEIL NYLANVIKRE NLSLPGKMEI MLSAQFDSAK NHLRYDTSAI   1450
TQNSNTEGLA TVSKIIKLDR K                                  1471