SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMTH1
Systematic nameYDR277C
DescriptionProtein MTH1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1   RINGSCFGRR1   
2   RINGSCFGRR1  Proteasomal Degradation
3K326;K333;K334;K336;K343  RINGSCFGRR1   
4         
* Blank: not determined, NA: not applicable.

References
[1]EvidenceProtein Stability Assay
MethodWestern blot
Reference"Grr1-dependent inactivation of Mth1 mediates glucose-induced dissociation of Rgt1 from HXT gene promoters."
Flick KM, Spielewoy N, Kalashnikova TI, Guaderrama M, Zhu Q, Chang HC, Wittenberg C.
Mol Biol Cell. 14(8):3230-41 (2003) [12925759]
[2]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Regulation and recognition of SCFGrr1 targets in the glucose and amino acid signaling pathways."
Spielewoy N, Flick K, Kalashnikova TI, Walker JR, Wittenberg C.
Mol Cell Biol. 24(20):8994-9005 (2004) [15456873]
[3]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Biochemical evidence for glucose-independent induction of HXT expression in Saccharomyces cerevisiae."
Pasula S, Jouandot D 2nd, Kim JH.
FEBS Lett. 581(17):3230-4 (2007) [17586499]
[4]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP35198
SGD YDR277C
Gene Ontology (GO)
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0015758glucose transportIMP:SGD.
Biological processGO:0007165signal transductionIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length433
Molecular weight49060

	---------+----------+----------+----------+----------+
MFVSPPPATS KNQVLQRRPL ESTNSNHGFA SSLQAIPENT MSGSDNASFQ   50
SLPLSMSSSQ STTSSRRENF VNAPPEYTDR ARDEIKKRLL ASSPSRRSHH   100
SSSMHSASRR SSVAESGSLL SDNASSYQSS IFSAPSTVHT QLTNDSSFSE   150
FPNHKLITRV SLDEALPKTF YDMYSPDILL ADPSNILCNG RPKFTKRELL   200
DWDLNDIRSL LIVEKLRPEW GNQLPEVITV GDNMPQFRLQ LLPLYSSDET   250
IIATLVHSDL YMEANLDYEF KLTSAKYTVA TARKRHEHIT GRNEAVMNLS   300
KPEWRNIIEN YLLNIAVEAQ CRFDFKQRCS EYKKWKLQQS NLKRPDMPPP   350
SIIPRKNSTE TKSLLKKALL KNIQLKNPNN NLDELMMRSS AATNQQGKNK   400
VSLSKEEKAT IWSQCQAQVY QRLGLDWQPD SVS                     433

	* Ubiquitinated lysine is denoted as red color.