SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMPG1
Systematic nameYDL055C
AliasPSA1, SRB1, VIG9
DescriptionMannose-1-phosphate guanyltransferase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP41940
SGD YDL055C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0004475mannose-1-phosphate guanylyltransferase act...IDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0000032cell wall mannoprotein biosynthetic processIMP:SGD.
Biological processGO:0009298GDP-mannose biosynthetic processIDA:SGD.
Biological processGO:0006486protein amino acid glycosylationIMP:SGD.
EC number 2.7.7.13

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length361
Molecular weight39566

	---------+----------+----------+----------+----------+
MKGLILVGGY GTRLRPLTLT VPKPLVEFGN RPMILHQIEA LANAGVTDIV   50
LAVNYRPEVM VETLKKYEKE YGVNITFSVE TEPLGTAGPL KLAEDVLKKD   100
NSPFFVLNSD VICEYPFKEL ADFHKAHGGK GTIVATKVDE PSKYGVIVHD   150
IATPNLIDRF VEKPKEFVGN RINAGLYILN PEVIDLIEMK PTSIEKETFP   200
ILVEEKQLYS FDLEGFWMDV GQPKDFLSGT VLYLNSLAKR QPKKLATGAN   250
IVGNALIDPT AKISSTAKIG PDVVIGPNVT IGDGVRITRS VVLCNSTIKN   300
HSLVKSTIVG WNSTVGQWCR LEGVTVLGDD VEVKDEIYIN GGKVLPHKSI   350
SDNVPKEAII M                                             361