SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMNT2
Systematic nameYGL257C
DescriptionAlpha-1,3-mannosyltransferase MNT2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP53059
SGD YGL257C
Gene Ontology (GO)
Molecular functionGO:0000033alpha-1,3-mannosyltransferase activityIDA:SGD.
Biological processGO:0006493protein amino acid O-linked glycosylationIDA:SGD.
EC number 2.4.1.-

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length558
Molecular weight64853

	---------+----------+----------+----------+----------+
MRRKNRLFIL VVLLGIVLVV YYSQLNSLDL VEPVQSSSSG NGGCWSYYEG   50
LTPGWLNDFY DVNQITPNPA KDVIELVTRI KIFFNCLQQV DGHNIQRLRD   100
IEKKLFPYIN FEKLETDESA FWHTTTRWNG EVYHASMLEF DPKNHQFLRS   150
KPINFDTGLS FWENWLHTVT QSGSKGIVIS ASDVQLNETI RLLKVLRFIK   200
NDYPIQIVHN ADLSQDSMKS IIKYARSLDT AEYPAQELWF LNVHSLLNPK   250
YSKKFTTYSN KWLALTFSSF EIPILMDSDT VPFVSIEKFY ELEEFQKTGV   300
LFFKDRVISD DLFESSELKI LREIVYGCIG LDLEDESKIH EQVEDPVVAQ   350
VLENMFIKKY KHHLESGLVI LHKGKHLFSM LTSIALQFSP IAEYFHGDKD   400
FFWLGELLSN NRFTFHPVDA SNIGQLGNVV SKESTGEFYQ ICSVQLSHTD   450
RDGSLLWLNG GLNICKKTSW EYDYEHRQRL NDMFQNADEL REYYASPVKL   500
EGIIIPDTSI SGWINSGECF LFNYCTLFKE GEFGKLIKFK EDEKLRLSQI   550
VDIWNKDI                                                 558