SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMLP1
Systematic nameYKR095W
DescriptionProtein MLP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]

Cross References
Swiss-Prot accessionQ02455
SGD YKR095W
Gene Ontology (GO)
Cellular componentGO:0005635nuclear envelopeIDA:SGD.
Cellular componentGO:0005654nucleoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0043021ribonucleoprotein bindingIGI:SGD.
Biological processGO:0006406mRNA export from nucleusIGI:SGD.
Biological processGO:0006606protein import into nucleusIGI:SGD.
Biological processGO:0045449regulation of transcriptionIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
337Phosphothreonine.
1678Phosphoserine.
1710Phosphoserine.

Sequence Information
Sequence length1875
Molecular weight218456

	---------+----------+----------+----------+----------+
MSDHDTPMES IQNGENSDER LNAIASFFGC SLEQVKSFDG DVVKHLNDKL   50
LQFNELKSEN LKVTVSFDEL KASSLKKIDG LKTEMENVIR ENDKIRKERN   100
DTFVKFESVE NEKMKLSSEL EFVKRKLDDL TEEKKETQSN QQRTLKILDE   150
RLKEIELVRV ENNRSNSECK KLRSTIMDLE TKQQGYITND LNSRTELERK   200
TQELTLLQSN NDWLEKELRS KNEQYLSYRQ KTDKVILDIR NELNRLRNDF   250
QMERTNNDVL KQKNNELSKS LQEKLLEIKG LSDSLNSEKQ EFSAEMSLKQ   300
RLVDLLESQL NAVKEELNSI RELNTAKVIA DDSKKQTPEN EDLLKELQLT   350
KEKLAQCEKE CLRLSSITDE ADEDNENLSA KSSSDFIFLK KQLIKERRTK   400
EHLQNQIETF IVELEHKVPI INSFKERTDM LENELNNAAL LLEHTSNEKN   450
AKVKELNAKN QKLVECENDL QTLTKQRLDL CRQIQYLLIT NSVSNDSKGP   500
LRKEEIQFIQ NIMQEDDSTI TESDSQKVVT ERLVEFKNII QLQEKNAELL   550
KVVRNLADKL ESKEKKSKQS LQKIESETVN EAKEAIITLK SEKMDLESRI   600
EELQKELEEL KTSVPNEDAS YSNVTIKQLT ETKRDLESQV QDLQTRISQI   650
TRESTENMSL LNKEIQDLYD SKSDISIKLG KEKSSRILAE ERFKLLSNTL   700
DLTKAENDQL RKRFDYLQNT ILKQDSKTHE TLNEYVSCKS KLSIVETELL   750
NLKEEQKLRV HLEKNLKQEL NKLSPEKDSL RIMVTQLQTL QKEREDLLEE   800
TRKSCQKKID ELEDALSELK KETSQKDHHI KQLEEDNNSN IEWYQNKIEA   850
LKKDYESVIT SVDSKQTDIE KLQYKVKSLE KEIEEDKIRL HTYNVMDETI   900
NDDSLRKELE KSKINLTDAY SQIKEYKDLY ETTSQSLQQT NSKLDESFKD   950
FTNQIKNLTD EKTSLEDKIS LLKEQMFNLN NELDLQKKGM EKEKADFKKR   1000
ISILQNNNKE VEAVKSEYES KLSKIQNDLD QQTIYANTAQ NNYEQELQKH   1050
ADVSKTISEL REQLHTYKGQ VKTLNLSRDQ LENALKENEK SWSSQKESLL   1100
EQLDLSNSRI EDLSSQNKLL YDQIQIYTAA DKEVNNSTNG PGLNNILITL   1150
RRERDILDTK VTVAERDAKM LRQKISLMDV ELQDARTKLD NSRVEKENHS   1200
SIIQQHDDIM EKLNQLNLLR ESNITLRNEL ENNNNKKKEL QSELDKLKQN   1250
VAPIESELTA LKYSMQEKEQ ELKLAKEEVH RWKKRSQDIL EKHEQLSSSD   1300
YEKLESEIEN LKEELENKER QGAEAEEKFN RLRRQAQERL KTSKLSQDSL   1350
TEQVNSLRDA KNVLENSLSE ANARIEELQN AKVAQGNNQL EAIRKLQEDA   1400
EKASRELQAK LEESTTSYES TINGLNEEIT TLKEEIEKQR QIQQQLQATS   1450
ANEQNDLSNI VESMKKSFEE DKIKFIKEKT QEVNEKILEA QERLNQPSNI   1500
NMEEIKKKWE SEHEQEVSQK IREAEEALKK RIRLPTEEKI NKIIERKKEE   1550
LEKEFEEKVE ERIKSMEQSG EIDVVLRKQL EAKVQEKQKE LENEYNKKLQ   1600
EELKDVPHSS HISDDERDKL RAEIESRLRE EFNNELQAIK KKSFDEGKQQ   1650
AMMKTTLLER KLAKMESQLS ETKQSAESPP KSVNNVQNPL LGLPRKIEEN   1700
SNSPFNPLLS GEKLLKLNSK SSSGGFNPFT SPSPNKHLQN DNDKRESLAN   1750
KTDPPTHLEP SFNIPASRGL ISSSSTLSTD TNDEELTSNN PAQKDSSNRN   1800
VQSEEDTEKK KEGEPVKRGE AIEEQTKSNK RPIDEVGELK NDEDDTTENI   1850
NESKKIKTED EEEKETDKVN DENSI                              1875