SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMLF3
Systematic nameYNL074C
AliasYMK1
DescriptionProtein MLF3

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32047
SGD YNL074C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Biological processGO:0042493response to drugIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
8Phosphoserine.
74Phosphoserine.
78Phosphoserine.
156Phosphoserine.
157Phosphoserine.
160Phosphoserine.
171Phosphoserine.
189Phosphoserine.
227Phosphotyrosine.
228Phosphoserine.
257Phosphoserine.
293Phosphoserine.
297Phosphoserine.
320Phosphoserine.
322Phosphoserine.

Sequence Information
Sequence length452
Molecular weight49210

	---------+----------+----------+----------+----------+
MCVYKSNSNN SNPSFIFERT VQEASSNDLF LQPPVSASNT SHSSRSNSFY   50
NLQTISPIPI SGSEVRTPSL RKNSNNVSSP LDNVIPTSRS ASNSTTSSLA   100
HQEYILNPIC NMQNHHHRRR TLENSVAPAL DASCSIVNDE NTDLSDVDMV   150
YSRRPSSAVS LNMALLARTN SATLPSSESS PASPDLKLSR SHSHSAATRP   200
TLNNINNTGM TTTTSNGEPN SRILRFYSYV DMLNDEKLAQ ANNTPTSRPP   250
MKSQAYSCPF ILKRSPPQAY SSSSATTTFS NPFIKTTELP ATSPYVSPQQ   300
SARQYSNNAN NNAKSPKNRS SSILFQRQSI LSNVDPVANM HKNPKFQIES   350
SDSEEEDLTM DMLDPSFPLS SSLRSSANLA SNPELATQTP LSTSSSYTAI   400
GKPMPLSTDP SYVSSSNTLS SEHELRVEKV SEVLKKKVSN GGFSTEFNSC   450
DT                                                       452