SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMLC2
Systematic nameYPR188C
DescriptionMyosin light chain 2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ06580
SGD YPR188C
Gene Ontology (GO)
Cellular componentGO:0000142cellular bud neck contractile ringIDA:SGD.
Molecular functionGO:0045159myosin II bindingIPI:SGD.
Biological processGO:0000916cytokinesis, contractile ring contractionIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length163
Molecular weight18521

	---------+----------+----------+----------+----------+
MDHSESLTFN QLTQDYINKL KDAFQMLDED EDGLISRGDL TKIYATLGKT   50
LTDEEWSKMV PDNDTSTAEV GEEGVSFPIF LSIMGKNLSQ FPEREELEES   100
LKAIGRGHDL NVPLNEVIDS LKEAGFENPE EEFAKLFKLF TTNQQATEER   150
TFRGKLFLDS ITD                                           163