SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMIP1
Systematic nameYOR330C
DescriptionDNA polymerase gamma

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP15801
SGD YOR330C
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0003895gamma DNA-directed DNA polymerase activityTAS:SGD.
Biological processGO:0006264mitochondrial DNA replicationIDA:SGD.
EC number 2.7.7.7

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1254
Molecular weight143502

	---------+----------+----------+----------+----------+
MTKLMVRSEC MLRMVRRRPL RVQFCARWFS TKKNTAEAPR INPVGIQYLG   50
ESLQRQVFGS CGGKDEVEQS DKLMELSKKS LKDHGLWGKK TLITDPISFP   100
LPPLQGRSLD EHFQKIGRFN SEPYKSFCED KFTEMVARPA EWLRKPGWVK   150
YVPGMAPVEV AYPDEELVVF DVETLYNVSD YPTLATALSS TAWYLWCSPF   200
ICGGDDPAAL IPLNTLNKEQ VIIGHNVAYD RARVLEEYNF RDSKAFFLDT   250
QSLHIASFGL CSRQRPMFMK NNKKKEAEVE SEVHPEISIE DYDDPWLNVS   300
ALNSLKDVAK FHCKIDLDKT DRDFFASTDK STIIENFQKL VNYCATDVTA   350
TSQVFDEIFP VFLKKCPHPV SFAGLKSLSK CILPTKLNDW NDYLNSSESL   400
YQQSKVQIES KIVQIIKDIV LLKDKPDFYL KDPWLSQLDW TTKPLRLTKK   450
GVPAKCQKLP GFPEWYRQLF PSKDTVEPKI TIKSRIIPIL FKLSWENSPV   500
IWSKESGWCF NVPHEQVETY KAKNYVLADS VSQEEEEIRT HNLGLQCTGV   550
LFKVPHPNGP TFNCTNLLTK SYNHFFEKGV LKSESELAHQ ALQINSSGSY   600
WMSARERIQS QFVVPSCKFP NEFQSLSAKS SLNNEKTNDL AIIIPKIVPM   650
GTITRRAVEN AWLTASNAKA NRIGSELKTQ VKAPPGYCFV GADVDSEELW   700
IASLVGDSIF NVHGGTAIGW MCLEGTKNEG TDLHTKTAQI LGCSRNEAKI   750
FNYGRIYGAG AKFASQLLKR FNPSLTDEET KKIANKLYEN TKGKTKRSKL   800
FKKFWYGGSE SILFNKLESI AEQETPKTPV LGCGITYSLM KKNLRANSFL   850
PSRINWAIQS SGVDYLHLLC CSMEYIIKKY NLEARLCISI HDEIRFLVSE   900
KDKYRAAMAL QISNIWTRAM FCQQMGINEL PQNCAFFSQV DIDSVIRKEV   950
NMDCITPSNK TAIPHGEALD INQLLDKSNS KLGKPNLDID SKVSQYAYNY   1000
REPVFEEYNK SYTPEFLKYF LAMQVQSDKR DVNRLEDEYL RECTSKEYAR   1050
DGNTAEYSLL DYIKDVEKGK RTKVRIMGSN FLDGTKNAKA DQRIRLPVNM   1100
PDYPTLHKIA NDSAIPEKQL LENRRKKENR IDDENKKKLT RKKNTTPMER   1150
KYKRVYGGRK AFEAFYECAN KPLDYTLETE KQFFNIPIDG VIDDVLNDKS   1200
NYKKKPSQAR TASSSPIRKT AKAVHSKKLP ARKSSTTNRN LVELERDITI   1250
SREY                                                     1254