SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMGA2
Systematic nameYIR033W
DescriptionProtein MGA2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI HECTNARSP5CDC48-UFD1/NPL4 Proteasomal Degradation
2   HECTNARSP5   
* Blank: not determined, NA: not applicable.

References
[1]EvidenceUb-conjugate Confirmation
MethodWestern blot
Reference"Rsp5p is required for ER bound Mga2p120 polyubiquitination and release of the processed/tethered transactivator Mga2p90."
Shcherbik N, Zoladek T, Nickels JT, Haines DS.
Curr Biol. 13(14):1227-33 (2003) [12867034]
[2]EvidenceHigh MW Product Confirmation
Methodfluorography (in vitro)
Reference"A single PXY motif located within the carboxyl terminus of Spt23p and Mga2p mediates a physical and functional interaction with ubiquitin ligase Rsp5p."
Shcherbik N, Kee Y, Lyon N, Huibregtse JM, Haines DS.
J Biol Chem. 279(51):53892-8 (2004) [15466864]

Cross References
Swiss-Prot accessionP40578
SGD YIR033W
Gene Ontology (GO)
Cellular componentGO:0005789endoplasmic reticulum membraneIDA:SGD.
Molecular functionGO:0016563transcriptional activator activityIMP:SGD.
Biological processGO:0006631fatty acid metabolic processIMP:SGD.
Biological processGO:0045944positive regulation of transcription from R...IMP:SGD.
Biological processGO:0009409response to coldIEP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
467Phosphoserine.

Sequence Information
Sequence length1113
Molecular weight127055

	---------+----------+----------+----------+----------+
MQQNSEFLTE TPGSDPHISQ LHANSVMESQ LLDDFLLNGS PMYQDDSMAH   50
INIDEGANFQ NFIKTDEGDS PNLLSFEGIG NNTHVNQNVS TPLEEEMESN   100
RALKEEEEDE HENKVFNEKN IGNPAHDEIV FGRKETIQSV YINPLDYLKV   150
NAAQLPLDVE VSGLPQVSRV ENQLKLKVKI TSETPLNQSM LYLPSDSISR   200
EKFYLKKNIE DFSEDFKKNL LYINAFVLCA VSNRTTNVCT KCVKREQRRA   250
ARRKSGIADN LLWCNNINRR LVVFNNKQVF PIMKTFDNVK EFELTTRLVC   300
YCRHHKANNG FVILFTITDW QNRLLGKFTT TPIMITDRKP ANMDTTKFNN   350
TTTSSRRQLT EEESTTEYYS TDNNQLSKDE NMPFQYTYQH NPYDNDSQMN   400
NIPLKDKNVP FPYSISQQTD LLQNNNLSLN LSLPNQHIPS PTSMSEEGSE   450
SFNYHHRDND NPVRTISLTN IEQQSQLNQR KRARNNLEND IGKPLFKHSF   500
SNSISATNTM NPALHSMQDF SMKNNNNNLP SINRVIPSQG PINGGIEVTL   550
LGCNFKDGLS VKFGSNLALS TQCWSETTIV TYLPPAAYAG QVFVSITDTN   600
NENNNDDLPQ EIEINDNKKA IFTYVDDTDR QLIELALQIV GLKMNGKLED   650
ARNIAKRIVG NDSPDSGTNG NSCSKSTGPS PNQHSMNLNT SVLYSDEVLI   700
QKVIKSLNIN SNISICDSLG RTLLHLACLK NYSSLVYTLI KKGARVNDID   750
SFGLTPLHFA CISGDPKIIK MLLNCKVNYS LRSHNGLTAR EVFIANHIHS   800
KEIDKKQDNR DNHKFVHNDT YISEVLSLFE EFQNGTKFTD SVETDSNYSI   850
SRKYSQSSFN SSLLDNESLN ENLFESQSMI NPTSMEIQHP TLQLFENSSY   900
SEYDQSDFEE DGDEDLFVTD EVEKPGVACR EEQSELLDIG SSANEPEEDN   950
GSTSLWNRVL HRINDDLPKY EDLFPLSWGK DDKLKTTNQD SIVEQSASNI   1000
ENSENSEEED YEEEEEFLKK QFNRFFQNKQ NFRNDKMLIF FWIPLTLLLL   1050
TWFIMYKFGN QDSSINHISE LISEYLRIAL AKFLLGNERM KTAFRSKLSN   1100
LQTTRMLNDL IVS                                           1113