SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameMFB1
Systematic nameYDR219C
DescriptionMitochondrial F-box protein MFB1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionQ04922
SGD YDR219C
Gene Ontology (GO)
Cellular componentGO:0031315extrinsic to mitochondrial outer membraneIDA:SGD.
Biological processGO:0007005mitochondrion organization and biogenesisIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length465
Molecular weight53168

	---------+----------+----------+----------+----------+
MTLFSCSVQM PLEERSLTNL PLNLLFRILS HLDMNDLQNI GKTCTLLRML   50
ANENIVYRNA VIGSNGNMWW TKNVLVDVFD VLNFNRKAMK TLNSHNISLV   100
ASLRNVQRKY KLGVIDPARK TISYRTNEVE SKEKGSVKDL NMDLNEPTEI   150
TREQIAHTAI LQGMNQFIEL NDKAFRTHSA DSDDTYIEEN NGEIHSLHGL   200
EKNTTFEEDL VKKPPFIPSP TFSNYSRSST NSVFSSSSPK LLDDDWNNIT   250
MDFTKSRDPD YKEMTPTSTE SSDSITRLRK SNKVKDKAEL FEKLIFRDSR   300
PLKTKKKDNP RLKLSSSLSA NDEDFRKIIS PPSDILPKVG RRSVSRGYLE   350
EIERHYPDFN GETTNPLAIK RVNSTKIANY EQLIIKENSS NCKGITEKND   400
ENKFQRSHTS PVIELSKPHQ RSKLKAVVTD GNKICYRKIE LDNPSGSNTN   450
DHVIKRLDAN TDFNI                                         465