SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


Main Search Enzymes Substrates Ubiquitination types BLAST Feedback HELP

Substrate Information

General Information
Standard nameMET6
Systematic nameYER091C
Description5-methyltetrahydropteroyltriglutamate--homocysteine methyltransferase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP05694
SGD YER091C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:00038715-methyltetrahydropteroyltriglutamate-homoc...IMP:SGD.
Biological processGO:0009086methionine biosynthetic processIMP:SGD.
EC number 2.1.1.14

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
89Phosphoserine.
706Phosphoserine.

Sequence Information
Sequence length767
Molecular weight85860

	---------+----------+----------+----------+----------+
MVQSAVLGFP RIGPNRELKK ATEGYWNGKI TVDELFKVGK DLRTQNWKLQ   50
KEAGVDIIPS NDFSFYDQVL DLSLLFNVIP DRYTKYDLSP IDTLFAMGRG   100
LQRKATETEK AVDVTALEMV KWFDSNYHYV RPTFSKTTQF KLNGQKPVDE   150
FLEAKELGIH TRPVLLGPVS YLFLGKADKD SLDLEPLSLL EQLLPLYTEI   200
LSKLASAGAT EVQIDEPVLV LDLPANAQAA IKKAYTYFGE QSNLPKITLA   250
TYFGTVVPNL DAIKGLPVAA LHVDFVRAPE QFDEVVAAIG NKQTLSVGIV   300
DGRNIWKNDF KKSSAIVNKA IEKLGADRVV VATSSSLLHT PVDLNNETKL   350
DAEIKGFFSF ATQKLDEVVV ITKNVSGQDV AAALEANAKS VESRGKSKFI   400
HDAAVKARVA SIDEKMSTRA APFEQRLPEQ QKVFNLPLFP TTTIGSFPQT   450
KDIRINRNKF NKGTISAEEY EKFINSEIEK VIRFQEEIGL DVLVHGEPER   500
NDMVQYFGEQ INGYAFTVNG WVQSYGSRYV RPPIIVGDLS RPKAMSVKES   550
VYAQSITSKP VKGMLTGPIT CLRWSFPRDD VDQKTQAMQL ALALRDEVND   600
LEAAGIKVIQ VDEPALREGL PLREGTERSA YYTWAAEAFR VATSGVANKT   650
QIHSHFCYSD LDPNHIKALD ADVVSIEFSK KDDANYIAEF KNYPNHIGLG   700
LFDIHSPRIP SKDEFIAKIS TILKSYPAEK FWVNPDCGLK TRGWEETRLS   750
LTHMVEAAKY FREQYKN                                       767