SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMET10
Systematic nameYFR030W
DescriptionSulfite reductase [NADPH] flavoprotein component

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP39692
SGD YFR030W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmTAS:SGD.
Cellular componentGO:0009337sulfite reductase complex (NADPH)IDA:SGD.
Biological processGO:0042493response to drugIMP:SGD.
Biological processGO:0000103sulfate assimilationTAS:SGD.
EC number 1.8.1.2

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1035
Molecular weight114829

	---------+----------+----------+----------+----------+
MPVEFATNPF GEAKNATSLP KYGTPVTAIS SVLFNNVDSI FAYKSFSQPD   50
LLHQDLKKWS EKRGNESRGK PFFQELDIRS GAGLAPLGFS HGLKNTTAIV   100
APGFSLPYFI NSLKTVSHDG KFLLNVGALN YDNATGSVTN DYVTALDAAS   150
KLKYGVVTPI SANEVQSVAL LALAIATFSN NSGAINLFDG LNYSKTVLPL   200
VESVPEASIL AKLSKVIAPD AAFDDVLDKF NELTGLRLHN FQYFGAQDAE   250
TVFITYGSLE SELFNSAISG NNSKIGLINV RVPLPFNVAK FVTHVPSTTK   300
QIVVIGQTLD GSSPSFLRSQ VSAALFYHGR TSISVSEYIY QPDFIWSPKA   350
VKSIVSSFIP EFTYNADSSF GEGFIYWASD KSINIDVASK LVKALSLEDG   400
KFVSLRTKFD NLANAGTFQA QFVTSKEQIP VSNIDSTKLS VVEDVSLLKH   450
LDVAATVAEQ GSIALVSQKA VKDLDLNSVE SYVKNLGIPE SFLISIAKKN   500
IKLFIIDGET TNDESKLSLF IQAVFWKLAF GLDVAECTNR IWKSIDSGAD   550
ISAASISEFL TGAFKNFLSE VPLALYTKFS EINIEKKEDE EEPAALPIFV   600
NETSFLPNNS TIEEIPLPET SEISDIAKKL SFKEAYEVEN KLRPDLPVKN   650
FVVKVKENRR VTPADYDRYI FHIEFDISGT GMTYDIGEAL GIHARNNESL   700
VKEFLTFYGL NESDVVLVPN KDNHHLLETR TVLQAFVENL DIFGKPPKRF   750
YESLIPYASN EEEKKKLEDL VTPAGAVDLK RFQDVEYYTY ADIFELFPSV   800
RPSLEELVTI IEPLKRREYS IASSQKVHPN EVHLLIVVVD WVDNKGRKRY   850
GQASKYISDL AVGSELVVSV KPSVMKLPPS PKQPVIMSGL GTGLAPFKAI   900
VEEKLWQKQQ GYEIGEVFLY LGSRHKREEY LYGELWEAYK DAGIITHIGA   950
AFSRDQPQKI YIQDRIKENL DELKTAMIDN KGSFYLCGPT WPVPDITQAL   1000
QDILAKDAEE RGIKVDLDAA IEELKEASRY ILEVY                   1035