SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMEP2
Systematic nameYNL142W
AliasAMT2
DescriptionAmmonium transporter MEP2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP41948
SGD YNL142W
Gene Ontology (GO)
Cellular componentGO:0005886plasma membraneIDA:SGD.
Molecular functionGO:0008519ammonium transporter activityIDA:SGD.
Biological processGO:0015696ammonium transportIMP:SGD.
Biological processGO:0019740nitrogen utilizationIMP:SGD.
Biological processGO:0007124pseudohyphal growthIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length499
Molecular weight53401

	---------+----------+----------+----------+----------+
MSYNFTGTPT GEGTGGNSLT TDLNTQFDLA NMGWIGVASA GVWIMVPGIG   50
LLYSGLSRKK HALSLLWASM MASAVCIFQW FFWGYSLAFS HNTRGNGFIG   100
TLEFFGFRNV LGAPSSVSSL PDILFAVYQG MFAAVTGALM LGGACERARL   150
FPMMVFLFLW MTIVYCPIAC WVWNAEGWLV KLGSLDYAGG LCVHLTSGHG   200
GLVYALILGK RNDPVTRKGM PKYKPHSVTS VVLGTVFLWF GWMFFNGGSA   250
GNATIRAWYS IMSTNLAAAC GGLTWMVIDY FRCGRKWTTV GLCSGIIAGL   300
VGITPAAGFV PIWSAVVIGV VTGAGCNLAV DLKSLLRIDD GLDCYSIHGV   350
GGCIGSVLTG IFAADYVNAT AGSYISPIDG GWINHHYKQV GYQLAGICAA   400
LAWTVTVTSI LLLTMNAIPF LKLRLSADEE ELGTDAAQIG EFTYEESTAY   450
IPEPIRSKTS AQMPPPHENI DDKIVGNTDA EKNSTPSDAS STKNTDHIV    499