SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMEC1
Systematic nameYBR136W
AliasESR1, SAD3
DescriptionSerine/threonine-protein kinase MEC1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38111
SGD YBR136W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005634nucleusIC:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004672protein kinase activityIDA:SGD.
Biological processGO:0000077DNA damage checkpointTAS:SGD.
Biological processGO:0000076DNA replication checkpointIMP:SGD.
Biological processGO:0007131meiotic recombinationIMP:SGD.
Biological processGO:0000722telomere maintenance via recombinationIGI:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
38Phosphoserine, by ATM or ATR.

Sequence Information
Sequence length2368
Molecular weight273342

	---------+----------+----------+----------+----------+
MESHVKYLDE LILAIKDLNS GVDSKVQIKK VPTDPSSSQE YAKSLKILNT   50
LIRNLKDQRR NNIMKNDTIF SKTVSALALL LEYNPFLLVM KDSNGNFEIQ   100
RLIDDFLNIS VLNYDNYHRI WFMRRKLGSW CKACVEFYGK PAKFQLTAHF   150
ENTMNLYEQA LTEVLLGKTE LLKFYDTLKG LYILLYWFTS EYSTFGNSIA   200
FLDSSLGFTK FDFNFQRLIR IVLYVFDSCE LAALEYAEIQ LKYISLVVDY   250
VCNRTISTAL DAPALVCCEQ LKFVLTTMHH FLDNKYGLLD NDPTMAKGIL   300
RLYSLCISND FSKCFVDHFP IDQWADFSQS EHFPFTQLTN KALSIVYFDL   350
KRRSLPVEAL KYDNKFNIWV YQSEPDSSLK NVTSPFDDRY KQLEKLRLLV   400
LKKFNKTERG TLLKYRVNQL SPGFFQRAGN DFKLILNEAS VSIQTCFKTN   450
NITRLTSWTV ILGRLACLES EKFSGTLPNS TKDMDNWYVC HLCDIEKTGN   500
PFVRINPNRP EAAGKSEIFR ILHSNFLSHP NIDEFSESLL SGILFSLHRI   550
FSHFQPPKLT DGNGQINKSF KLVQKCFMNS NRYLRLLSTR IIPLFNISDS   600
HNSEDEHTAT LIKFLQSQKL PVVKENLVIA WTQLTLTTSN DVFDTLLLKL   650
IDIFNSDDYS LRIMMTLQIK NMAKILKKTP YQLLSPILPV LLRQLGKNLV   700
ERKVGFQNLI ELLGYSSKTI LDIFQRYIIP YAIIQYKSDV LSEIAKIMCD   750
GDTSLINQMK VNLLKKNSRQ IFAVALVKHG LFSLDILETL FLNRAPTFDK   800
GYITAYLPDY KTLAEITKLY KNSVTKDASD SENANMILCS LRFLITNFEK   850
DKRHGSKYKN INNWTDDQEQ AFQKKLQDNI LGIFQVFSSD IHDVEGRTTY   900
YEKLRVINGI SFLIIYAPKK SIISALAQIS ICLQTGLGLK EVRYEAFRCW   950
HLLVRHLNDE ELSTVIDSLI AFILQKWSEF NGKLRNIVYS ILDTLIKEKS   1000
DLILKLKPYT TLALVGKPEL GILARDGQFA RMVNKIRSTT DLIPIFANNL   1050
KSSNKYVINQ NLDDIEVYLR RKQTERSIDF TPKKVGQTSD ITLVLGALLD   1100
TSHKFRNLDK DLCEKCAKCI SMIGVLDVTK HEFKRTTYSE NEVYDLNDSV   1150
QTIKFLIWVI NDILVPAFWQ SENPSKQLFV ALVIQESLKY CGLSSESWDM   1200
NHKELYPNEA KLWEKFNSVS KTTIYPLLSS LYLAQSWKEY VPLKYPSNNF   1250
KEGYKIWVKR FTLDLLKTGT TENHPLHVFS SLIREDDGSL SNFLLPYISL   1300
DIIIKAEKGT PYADILNGII IEFDSIFTCN LEGMNNLQVD SLRMCYESIF   1350
RVFEYCKKWA TEFKQNYSKL HGTFIIKDTK TTNMLLRIDE FLRTTPSDLL   1400
AQRSLETDSF ERSALYLEQC YRQNPHDKNQ NGQLLKNLQI TYEEIGDIDS   1450
LDGVLRTFAT GNLVSKIEEL QYSENWKLAQ DCFNVLGKFS DDPKTTTRML   1500
KSMYDHQLYS QIISNSSFHS SDGKISLSPD VKEWYSIGLE AANLEGNVQT   1550
LKNWVEQIES LRNIDDREVL LQYNIAKALI AISNEDPLRT QKYIHNSFRL   1600
IGTNFITSSK ETTLLKKQNL LMKLHSLYDL SFLSSAKDKF EYKSNTTILD   1650
YRMERIGADF VPNHYILSMR KSFDQLKMNE QADADLGKTF FTLAQLARNN   1700
ARLDIASESL MHCLERRLPQ AELEFAEILW KQGENDRALK IVQEIHEKYQ   1750
ENSSVNARDR AAVLLKFTEW LDLSNNSASE QIIKQYQDIF QIDSKWDKPY   1800
YSIGLYYSRL LERKKAEGYI TNGRFEYRAI SYFLLAFEKN TAKVRENLPK   1850
VITFWLDIAA ASISEAPGNR KEMLSKATED ICSHVEEALQ HCPTYIWYFV   1900
LTQLLSRLLH SHQSSAQIIM HILLSLAVEY PSHILWYITA LVNSNSSKRV   1950
LRGKHILEKY RQHSQNPHDL VSSALDLTKA LTRVCLQDVK SITSRSGKSL   2000
EKDFKFDMNV APSAMVVPVR KNLDIISPLE SNSMRGYQPF RPVVSIIRFG   2050
SSYKVFSSLK KPKQLNIIGS DGNIYGIMCK KEDVRQDNQY MQFATTMDFL   2100
LSKDIASRKR SLGINIYSVL SLREDCGILE MVPNVVTLRS ILSTKYESLK   2150
IKYSLKSLHD RWQHTAVDGK LEFYMEQVDK FPPILYQWFL ENFPDPINWF   2200
NARNTYARSY AVMAMVGHIL GLGDRHCENI LLDIQTGKVL HVDFDCLFEK   2250
GKRLPVPEIV PFRLTPNLLD ALGIIGTEGT FKKSSEVTLA LMRKNEVALM   2300
NVIETIMYDR NMDHSIQKAL KVLRNKIRGI DPQDGLVLSV AGQTETLIQE   2350
ATSEDNLSKM YIGWLPFW                                      2368