SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMDR1
Systematic nameYGR100W
AliasGYP2, MIC1
DescriptionGTPase-activating protein GYP2

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP53258
SGD YGR100W
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Cellular componentGO:0005625soluble fractionTAS:SGD.
Molecular functionGO:0042802identical protein bindingIPI:IntAct.
Molecular functionGO:0005097Rab GTPase activator activityIDA:SGD.
Biological processGO:0006886intracellular protein transportIGI:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
764Phosphoserine.
862Phosphoserine.
871Phosphoserine.

Sequence Information
Sequence length950
Molecular weight109259

	---------+----------+----------+----------+----------+
MSFFDSLRQK APFLDKLADS FTPTLTRDEK FRLKYKLPAN ENILEDTNAE   50
VSFATSIKDG KGHSDRVNNK GRKTAYVYSG RLFLTPHFLV FRDAFDHSSC   100
VLILNISTIK RVERSPSESY EFALLVTLYT GAKVLIQFIG IRYRSEQFCD   150
KLKLNLKENI PNAKTLPAFL ETSYSEFLIA KNILGKKDIT VPRAGLGQHF   200
KYPGNPTMVK EKAKLRLWFD YFRENGRNLA VVQTPMFRKL IRIGVPNRMR   250
GEIWELCSGA MYMRYANSGE YERILNENAG KTSQAIDEIE KDLKRSLPEY   300
SAYQTEEGIQ RLRNVLTAYS WKNPDVGYCQ AMNIVVAGFL IFMSEEQAFW   350
CLCNLCDIYV PGYYSKTMYG TLLDQRVFES FVEDRMPVLW EYILQHDIQL   400
SVVSLPWFLS LFFTSMPLEY AVRIMDIFFM NGSITLFQVA LAVLKINADD   450
ILQADDDGMF IAIIKHYFQT LGQSAHPDSS DIKYRQITKF QELLVTAFKE   500
FSVISEEMAM HARHKYEKGI FQNIETFMKR TQLRHMPKTF NLSSDDLSNI   550
YDMFYQSIET YKISMGTGSS NMGFEVFIQF LSKFCDSCRP CEKDKDPAFR   600
KQKRNFLQRL FDNWDSAHIG ELTLNDVVTG LDKLVTVDLL QAINYFFSLY   650
DTDGDGELHR EEVLQLSEGL LLLTEPWKSG RYVDLLTKKR IEDDIAENII   700
KESGGEIATM NQIELPTGVT IDEEKYKVEQ AERYLKAASN FLQRSFEYAK   750
AVDLAEEVNL IDLSDDEGEE KRTVKQKQLE SIKANAALDP THPKVIDLPT   800
FRMIILADET YELFFSNTLR SSVHVDEHVN IDNKNKVLRS MFDGILADGK   850
RVAEQVRRRV DSVATRSSIA SVESTPTAAA SSITTKEEKY DDLDDFTSEH   900
QPENEELLQS SWFEIDDANE TSTKAIQERS FEPLSANSSE EKSNLIEFEA   950