SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameMCH4
Systematic nameYOL119C
DescriptionProbable transporter MCH4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2 MULTI    RPN10 Proteasomal Degradation
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ08268
SGD YOL119C
Gene Ontology (GO)
Cellular componentGO:0000329membrane of vacuole with cell cycle-correla...IDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
76Phosphothreonine.

Sequence Information
Sequence length501
Molecular weight54707

	---------+----------+----------+----------+----------+
MLNIPIIANS KRFLFSKDHE AQSTRDHDVE LETREGPSSG YNPNFNAADA   50
ILKKNSDQVD LDVNKLTNVT SRVLNTPEAS LIYDDDREFP DGGLKAWLVV   100
FGAFMGLVPV FGLINSLGAI ESYISKHQLA NISSSTISWI FSLYLAISFL   150
SCILSGGYFD RNGSIGLMCT GTVIYAGGLF ALANCKSVWQ FILAFSVCSG   200
LGTGILMTPL IGTVATWFLK RRGIATSIST MGGSIGGIVF PIMLRKLYKE   250
VGFQWAIRIL SFICLTCLIC ASVLARERTK PVVQPFKSKA EVAKWYISSV   300
FNWRYFLEGK FLFVAIGASF AESSLTSCAT YLASYSMTRG NTENVAYTMI   350
TASNAVGILG RYIPGYFADK FIGRFNVEII TISMAALFNF VMWLPFGGNT   400
KVLWAYVCLW GFSTGSILSL TPVCIGQISK TTDFGKRYAT VYLLQALVTI   450
PVLPIGGTLI GKGTVANYNH FIIFNSALMA AGAACYIISR HICVGAKLCK   500
F                                                        501