SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLYS21
Systematic nameYDL131W
DescriptionHomocitrate synthase, mitochondrial

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionQ12122
SGD YDL131W
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0019878lysine biosynthetic process via aminoadipic...TAS:SGD.
EC number 2.3.3.14

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
409Phosphoserine.
410Phosphothreonine.

Sequence Information
Sequence length440
Molecular weight48594

	---------+----------+----------+----------+----------+
MSENNEFQSV TESTTAPTTS NPYGPNPADY LSNVKNFQLI DSTLREGEQF   50
ANAFFDTEKK IEIARALDDF GVDYIELTSP VASEQSRKDC EAICKLGLKA   100
KILTHIRCHM DDARVAVETG VDGVDVVIGT SKFLRQYSHG KDMNYIAKSA   150
VEVIEFVKSK GIEIRFSSED SFRSDLVDLL NIYKTVDKIG VNRVGIADTV   200
GCANPRQVYE LIRTLKSVVS CDIECHFHND TGCAIANAYT ALEGGARLID   250
VSVLGIGERN GITPLGGLMA RMIVAAPDYV RSKYKLHKIR DIENLVADAV   300
EVNIPFNNPI TGFCAFTHKA GIHAKAILAN PSTYEILDPH DFGMKRYIHF   350
ANRLTGWNAI KSRVDQLNLN LTDDQIKEVT AKIKKLGDVR PLNIDDVDSI   400
IKDFHAELST PLLKPVNKGT DDDNIDISNG HVSKKAKVTK               440