SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLYS20
Systematic nameYDL182W
DescriptionHomocitrate synthase, cytosolic isozyme

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP48570
SGD YDL182W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004410homocitrate synthase activityIEP:SGD.
Biological processGO:0019878lysine biosynthetic process via aminoadipic...TAS:SGD.
EC number 2.3.3.14

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
395Phosphoserine.
396Phosphothreonine.
401Phosphoserine.
410Phosphoserine.

Sequence Information
Sequence length428
Molecular weight47099

	---------+----------+----------+----------+----------+
MTAAKPNPYA AKPGDYLSNV NNFQLIDSTL REGEQFANAF FDTEKKIEIA   50
RALDDFGVDY IELTSPVASE QSRKDCEAIC KLGLKAKILT HIRCHMDDAK   100
VAVETGVDGV DVVIGTSKFL RQYSHGKDMN YIAKSAVEVI EFVKSKGIEI   150
RFSSEDSFRS DLVDLLNIYK TVDKIGVNRV GIADTVGCAN PRQVYELIRT   200
LKSVVSCDIE CHFHNDTGCA IANAYTALEG GARLIDVSVL GIGERNGITP   250
LGGLMARMIV AAPDYVKSKY KLHKIRDIEN LVADAVEVNI PFNNPITGFC   300
AFTHKAGIHA KAILANPSTY EILDPHDFGM KRYIHFANRL TGWNAIKARV   350
DQLNLNLTDD QIKEVTAKIK KLGDVRSLNI DDVDSIIKNF HAEVSTPQVL   400
SAKKNKKNDS DVPELATIPA AKRTKPSA                           428