SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLYS2
Systematic nameYBR115C
DescriptionL-aminoadipate-semialdehyde dehydrogenase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP07702
SGD YBR115C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0004043L-aminoadipate-semialdehyde dehydrogenase a...IDA:SGD.
Biological processGO:0019878lysine biosynthetic process via aminoadipic...IDA:SGD.
EC number 1.2.1.31

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length1392
Molecular weight155346

	---------+----------+----------+----------+----------+
MTNEKVWIEK LDNPTLSVLP HDFLRPQQEP YTKQATYSLQ LPQLDVPHDS   50
FSNKYAVALS VWAALIYRVT GDDDIVLYIA NNKILRFNIQ PTWSFNELYS   100
TINNELNKLN SIEANFSFDE LAEKIQSCQD LERTPQLFRL AFLENQDFKL   150
DEFKHHLVDF ALNLDTSNNA HVLNLIYNSL LYSNERVTIV ADQFTQYLTA   200
ALSDPSNCIT KISLITASSK DSLPDPTKNL GWCDFVGCIH DIFQDNAEAF   250
PERTCVVETP TLNSDKSRSF TYRDINRTSN IVAHYLIKTG IKRGDVVMIY   300
SSRGVDLMVC VMGVLKAGAT FSVIDPAYPP ARQTIYLGVA KPRGLIVIRA   350
AGQLDQLVED YINDELEIVS RINSIAIQEN GTIEGGKLDN GEDVLAPYDH   400
YKDTRTGVVV GPDSNPTLSF TSGSEGIPKG VLGRHFSLAY YFNWMSKRFN   450
LTENDKFTML SGIAHDPIQR DMFTPLFLGA QLYVPTQDDI GTPGRLAEWM   500
SKYGCTVTHL TPAMGQLLTA QATTPFPKLH HAFFVGDILT KRDCLRLQTL   550
AENCRIVNMY GTTETQRAVS YFEVKSKNDD PNFLKKLKDV MPAGKGMLNV   600
QLLVVNRNDR TQICGIGEIG EIYVRAGGLA EGYRGLPELN KEKFVNNWFV   650
EKDHWNYLDK DNGEPWRQFW LGPRDRLYRT GDLGRYLPNG DCECCGRADD   700
QVKIRGFRIE LGEIDTHISQ HPLVRENITL VRKNADNEPT LITFMVPRFD   750
KPDDLSKFQS DVPKEVETDP IVKGLIGYHL LSKDIRTFLK KRLASYAMPS   800
LIVVMDKLPL NPNGKVDKPK LQFPTPKQLN LVAENTVSET DDSQFTNVER   850
EVRDLWLSIL PTKPASVSPD DSFFDLGGHS ILATKMIFTL KKKLQVDLPL   900
GTIFKYPTIK AFAAEIDRIK SSGGSSQGEV VENVTANYAE DAKKLVETLP   950
SSYPSREYFV EPNSAEGKTT INVFVTGVTG FLGSYILADL LGRSPKNYSF   1000
KVFAHVRAKD EEAAFARLQK AGITYGTWNE KFASNIKVVL GDLSKSQFGL   1050
SDEKWMDLAN TVDIIIHNGA LVHWVYPYAK LRDPNVISTI NVMSLAAVGK   1100
PKFFDFVSST STLDTEYYFN LSDKLVSEGK PGILESDDLM NSASGLTGGY   1150
GQSKWAAEYI IRRAGERGLR GCIVRPGYVT GASANGSSNT DDFLLRFLKG   1200
SVQLGKIPDI ENSVNMVPVD HVARVVVATS LNPPKENELA VAQVTGHPRI   1250
LFKDYLYTLH DYGYDVEIES YSKWKKSLEA SVIDRNEENA LYPLLHMVLD   1300
NLPESTKAPE LDDRNAVASL KKDTAWTGVD WSNGIGVTPE EVGIYIAFLN   1350
KVGFLPPPTH NDKLPLPSIE LTQAQISLVA SGAGARGSSA AA           1392