SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLYS1
Systematic nameYIR034C
DescriptionSaccharopine dehydrogenase [NAD+, L-lysine-forming]

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI       
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]

Cross References
Swiss-Prot accessionP38998
SGD YIR034C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Molecular functionGO:0004754saccharopine dehydrogenase (NAD+, L-lysine-...TAS:SGD.
Biological processGO:0019878lysine biosynthetic process via aminoadipic...TAS:SGD.
EC number 1.5.1.7

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
2N-acetylalanine, partial.

Sequence Information
Sequence length373
Molecular weight41465

	---------+----------+----------+----------+----------+
MAAVTLHLRA ETKPLEARAA LTPTTVKKLI AKGFKIYVED SPQSTFNINE   50
YRQAGAIIVP AGSWKTAPRD RIIIGLKEMP ETDTFPLVHE HIQFAHCYKD   100
QAGWQNVLMR FIKGHGTLYD LEFLENDQGR RVAAFGFYAG FAGAALGVRD   150
WAFKQTHSDD EDLPAVSPYP NEKALVKDVT KDYKEALATG ARKPTVLIIG   200
ALGRCGSGAI DLLHKVGIPD ANILKWDIKE TSRGGPFDEI PQADIFINCI   250
YLSKPIAPFT NMEKLNNPNR RLRTVVDVSA DTTNPHNPIP IYTVATVFNK   300
PTVLVPTTAG PKLSVISIDH LPSLLPREAS EFFSHDLLPS LELLPQRKTA   350
PVWVRAKKLF DRHCARVKRS SRL                                373