SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLYP1
Systematic nameYNL268W
DescriptionLysine-specific permease

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP32487
SGD YNL268W
Gene Ontology (GO)
Cellular componentGO:0005739mitochondrionIDA:SGD.
Molecular functionGO:0015174basic amino acid transporter activityIDA:SGD.
Biological processGO:0015802basic amino acid transportIDA:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
75Phosphoserine.
79Phosphoserine.
90Phosphothreonine.

Sequence Information
Sequence length611
Molecular weight68090

	---------+----------+----------+----------+----------+
MGRFSNIITS NKWDEKQNNI GEQSMQELPE DQIEHEMEAI DPSNKTTPYS   50
IDEKQYNTKK KHGSLQGGAI ADVNSITNSL TRLQVVSHET DINEDEEEAH   100
YEDKHVKRAL KQRHIGMIAL GGTIGTGLFV GISTPLSNAG PVGSLIAYIF   150
MGTIVYFVTQ SLGEMATFIP VTSSITVFSK RFLSPAFGVS NGYMYWFNWA   200
ITYAVEVSVI GQVIEYWTDK VPLAAWIAIF WVIITLMNFF PVKVYGEFEF   250
WVASVKVLAI MGYLIYALII VCGGSHQGPI GFRYWRNPGA WGPGIISSDK   300
SEGRFLGWVS SLINAAFTYQ GTELVGITAG EAANPRKTVP RAINKVVFRI   350
VLFYIMSLFF IGLLVPYNDS RLSASSAVIA SSPFVISIQN AGTYALPDIF   400
NAVVLITVVS AANSNVYVGS RVLYSLARTG NAPKQFGYVT RQGVPYLGVV   450
CTAALGLLAF LVVNNNANTA FNWLINISTL AGLCAWLFIS LAHIRFMQAL   500
KHRGISRDDL PFKAKLMPYG AYYAAFFVTV IIFIQGFQAF CPFKVSEFFT   550
SYISLILLAV VFIGCQIYYK CRFIWKLEDI DIDSDRREIE AIIWEDDEPK   600
NLWEKFWAAV A                                             611