SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLRO1
Systematic nameYNR008W
DescriptionPhospholipid:diacylglycerol acyltransferase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]

Cross References
Swiss-Prot accessionP40345
SGD YNR008W
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumTAS:SGD.
Molecular functionGO:0046027phospholipid:diacylglycerol acyltransferase...IDA:SGD.
Biological processGO:0019915sequestering of lipidIDA:SGD.
Biological processGO:0019432triacylglycerol biosynthetic processIDA:SGD.
EC number 2.3.1.158

Additional Features
Other Post Translational Modifications (PTM)  

Sequence Information
Sequence length661
Molecular weight75393

	---------+----------+----------+----------+----------+
MGTLFRRNVQ NQKSDSDENN KGGSVHNKRE SRNHIHHQQG LGHKRRRGIS   50
GSAKRNERGK DFDRKRDGNG RKRWRDSRRL IFILGAFLGV LLPFSFGAYH   100
VHNSDSDLFD NFVNFDSLKV YLDDWKDVLP QGISSFIDDI QAGNYSTSSL   150
DDLSENFAVG KQLLRDYNIE AKHPVVMVPG VISTGIESWG VIGDDECDSS   200
AHFRKRLWGS FYMLRTMVMD KVCWLKHVML DPETGLDPPN FTLRAAQGFE   250
STDYFIAGYW IWNKVFQNLG VIGYEPNKMT SAAYDWRLAY LDLERRDRYF   300
TKLKEQIELF HQLSGEKVCL IGHSMGSQII FYFMKWVEAE GPLYGNGGRG   350
WVNEHIDSFI NAAGTLLGAP KAVPALISGE MKDTIQLNTL AMYGLEKFFS   400
RIERVKMLQT WGGIPSMLPK GEEVIWGDMK SSSEDALNNN TDTYGNFIRF   450
ERNTSDAFNK NLTMKDAINM TLSISPEWLQ RRVHEQYSFG YSKNEEELRK   500
NELHHKHWSN PMEVPLPEAP HMKIYCIYGV NNPTERAYVY KEEDDSSALN   550
LTIDYESKQP VFLTEGDGTV PLVAHSMCHK WAQGASPYNP AGINVTIVEM   600
KHQPDRFDIR GGAKSAEHVD ILGSAELNDY ILKIASGNGD LVEPRQLSNL   650
SQWVSQMPFP M                                             661