SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLEU1
Systematic nameYGL009C
Description3-isopropylmalate dehydratase

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1 MULTI    RPN10 Proteasomal Degradation
2         
3         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS
Reference"Analysis of polyubiquitin conjugates reveals that the Rpn10 substrate receptor contributes to the turnover of multiple proteasome targets."
Mayor T, Lipford JR, Graumann J, Smith GT, Deshaies RJ.
Mol Cell Proteomics. 4(6):741-51 (2005) [15699485]
[2]EvidencePurification & Protein ID
Methodpurification (HB-ubiquitin (a tandem affinity tag under denaturing conditions)), LC/MS
Reference"A tandem affinity tag for two-step purification under fully denaturing conditions: application in ubiquitin profiling and protein complex identification combined with in vivocross-linking."
Tagwerker C, Flick K, Cui M, Guerrero C, Dou Y, Auer B, Baldi P, Huang L, Kaiser P.
Mol Cell Proteomics. 5(4):737-48 (2006) [16432255]
[3]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP07264
SGD YGL009C
Gene Ontology (GO)
Cellular componentGO:0005625soluble fractionIDA:SGD.
EC number 4.2.1.33

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
82Phosphoserine.
488Phosphoserine.
494Phosphothreonine.
495Phosphoserine.
503Phosphoserine.
536Phosphothreonine.
748Phosphoserine.

Sequence Information
Sequence length779
Molecular weight85794

	---------+----------+----------+----------+----------+
MVYTPSKGPR TLYDKVFDAH VVHQDENGSF LLYIDRHLVH EVTSPQAFEG   50
LENAGRKVRR VDCTLATVDH NIPTESRKNF KSLDTFIKQT DSRLQVKTLE   100
NNVKQFGVPY FGMSDARQGI VHTIGPEEGF TLPGTTVVCG DSHTSTHGAF   150
GSLAFGIGTS EVEHVLATQT IIQAKSKNMR ITVNGKLSPG ITSKDLILYI   200
IGLIGTAGGT GCVIEFAGEA IEALSMEARM SMCNMAIEAG ARAGMIKPDE   250
TTFQYTKGRP LAPKGAEWEK AVAYWKTLKT DEGAKFDHEI NIEAVDVIPT   300
ITWGTSPQDA LPITGSVPDP KNVTDPIKKS GMERALAYMG LEPNTPLKSI   350
KVDKVFIGSC TNGRIEDLRS AAAVVRGQKL ASNIKLAMVV PGSGLVKKQA   400
EAEGLDKIFQ EAGFEWREAG CSICLGMNPD ILDAYERCAS TSNRNFEGRQ   450
GALSRTHLMS PAMAAAAGIA GHFVDIREFE YKDQDQSSPK VEVTSEDEKE   500
LESAAYDHAE PVQPEDAPQD IANDELKDIP VKSDDTPAKP SSSGMKPFLT   550
LEGISAPLDK ANVDTDAIIP KQFLKTIKRT GLKKGLFYEW RFRKDDQGKD   600
QETDFVLNVE PWREAEILVV TGDNFGCGSS REHAPWALKD FGIKSIIAPS   650
YGDIFYNNSF KNGLLPIRLD QQIIIDKLIP IANKGGKLCV DLPNQKILDS   700
DGNVLVDHFE IEPFRKHCLV NGLDDIGITL QKEEYISRYE ALRREKYSFL   750
EGGSKLLKFD NVPKRKAVTT TFDKVHQDW                          779