SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLDB19
Systematic nameYOR322C
DescriptionProtein LDB19

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1K486        
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS
Reference"A subset of membrane-associated proteins is ubiquitinated in response to mutations in the endoplasmic reticulum degradation machinery."
Hitchcock AL, Auld K, Gygi SP, Silver PA.
Proc Natl Acad Sci U S A. 100(22):12735-40 (2003) [14557538]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ12502
SGD YOR322C
Gene Ontology (GO)
Cellular componentGO:0005737cytoplasmIDA:SGD.
Biological processGO:0000723telomere maintenanceIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
93Phosphothreonine.

Sequence Information
Sequence length818
Molecular weight89826

	---------+----------+----------+----------+----------+
MAFSRLTSTH QSNHNGYSNS NKKGQSLPLT LSIDVESPPC VLYGSAMESS   50
GAVLSGLFTV TVVDPYSSAE DKSLKNTESN VSTTSKSLKR KSTFGSALSS   100
RLSSLSASTS NISPSTSSTS ISHSPTPANL RIMAGYTKIT ITSVTLSLVQ   150
KIHFHKPFVP NISSMQTCMN CKTKITNMKS WEIQSNTQDL SVGSHSYPFS   200
YLIPGSVPCS SSLGATAETQ VKYELIAVVT YIDPHRNSFS SGHSTPRKEG   250
SSSKKRLLQL AMPIAVTRSI PRGPDKNSLR VFPPTELTAA AVLPNVVYPK   300
STFPLEMKLD GVSSGDRRWR MRKLSWRIEE TTRVKAHACP VHKHELRQLE   350
EQVKIKESEK SKKPRSHIKR YGELGPQIRV AVNSLENMPS QRLPGEPGRE   400
QAPNSSGPAS TGNVGLDDEN PVNEDEEDQP GSEFIHPSDD ALRQELLMQQ   450
QRARQQQLQQ ELKNNSSLFT EEVRIISKGE MKSGWKTDFD NNGKIELVTE   500
IDCMGLNSGV SNPVMHASTL QTPSTGNKKP SINVACDIQD PNLGLYVSHI   550
LAVEIVVAEE TLQYANGQPI RKPNSKNKKE TNNNTMNVHN PDQRLAELSP   600
IFANRNTPKV RRMGPEDITP VNSNKSNHST NKEKASNGAS NSNIVSVPTG   650
AARVLRMQFR LTVTERSGLG ISWDEEVPPI YQDVELLSPP CYELSINNGI   700
KNKLYSTMST PVRSEDDFVG GSDEDIGNYE SQGLEPGPNV QEVTITQNKL   750
TIPPTAHHYQ PASSSQRSLT TVQSPPLESV VSVQGSVPFR GHVLTPHSTR   800
DIRIQNFSDF LDSNRITQ                                      818

	* Ubiquitinated lysine is denoted as red color.