SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLCB4
Systematic nameYOR171C
DescriptionSphingoid long chain base kinase 4

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1        Proteasomal Degradation
2         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (ubiquitin-chain by UBA domain-containing protein, 6xHis-ubiquitin (two-step)), LC/MS, validation (statistical quantitative analysis)
Reference"Quantitative profiling of ubiquitylated proteins reveals proteasome substrates and the substrate repertoire influenced by the Rpn10 receptor pathway."
Mayor T, Graumann J, Bryan J, MacCoss MJ, Deshaies RJ.
Mol Cell Proteomics. 6(11):1885-95 (2007) [17644757]
[2]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ12246
SGD YOR171C
Gene Ontology (GO)
Cellular componentGO:0005783endoplasmic reticulumIDA:SGD.
Cellular componentGO:0005794Golgi apparatusIDA:SGD.
Cellular componentGO:0005624membrane fractionIDA:SGD.
Cellular componentGO:0005886plasma membraneIDA:SGD.
Cellular componentGO:0005625soluble fractionIDA:SGD.
Molecular functionGO:0017050D-erythro-sphingosine kinase activityIDA:SGD.
Molecular functionGO:0005515protein bindingIPI:IntAct.
Biological processGO:0019722calcium-mediated signalingIMP:SGD.
Biological processGO:0006665sphingolipid metabolic processIDA:SGD.
EC number 2.7.1.-

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
451Phosphoserine, by PHO85.
454Phosphoserine.
455Phosphoserine, by PHO85.
468Phosphothreonine.

Sequence Information
Sequence length624
Molecular weight69639

	---------+----------+----------+----------+----------+
MVVQKKLRAI LTDEGVLIKS QSHHMFNKHG QLRSGDSLSL LSCLSCLDDG   50
TLSSDGGSFD EDDSLELLPL NTTIPFNRIL NAKYVNVGQK GFNNGKISSN   100
PFQTENLSSS SENDDVENHS LSNDKAPVSE SQSFPKKDKW DTKTNTVKVS   150
PDDSQDNSPS LGIKDNQQLI ELTFAVPKGH DVIPQKLTLL IDHVSRKSRA   200
NTGEENISSG TVEEILEKSY ENSKRNRSIL VIINPHGGKG TAKNLFLTKA   250
RPILVESGCK IEIAYTKYAR HAIDIAKDLD ISKYDTIACA SGDGIPYEVI   300
NGLYRRPDRV DAFNKLAVTQ LPCGSGNAMS ISCHWTNNPS YAALCLVKSI   350
ETRIDLMCCS QPSYMNEWPR LSFLSQTYGV IAESDINTEF IRWMGPVRFN   400
LGVAFNIIQG KKYPCEVFVK YAAKSKKELK VHFLENKDKN KGCLTFEPNP   450
SPNSSPDLLS KNNINNSTKD ELSPNFLNED NFKLKYPMTE PVPRDWEKMD   500
SELTDNLTIF YTGKMPYIAK DTKFFPAALP ADGTIDLVIT DARIPVTRMT   550
PILLSLDKGS HVLEPEVIHS KILAYKIIPK VESGLFSVDG EKFPLEPLQV   600
EIMPMLCKTL LRNGRYIDTE FESM                               624