SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameLAS17
Systematic nameYOR181W
DescriptionProline-rich protein LAS17

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionQ12446
SGD YOR181W
Gene Ontology (GO)
Cellular componentGO:0030479actin cortical patchIDA:SGD.
Molecular functionGO:0008092cytoskeletal protein bindingIDA:SGD.
Biological processGO:0007015actin filament organizationIDA:SGD.
Biological processGO:0008154actin polymerization and/or depolymerizationIDA:SGD.
Biological processGO:0007121bipolar cellular bud site selectionIMP:SGD.
Biological processGO:0006897endocytosisIMP:SGD.
Biological processGO:0006970response to osmotic stressIMP:SGD.
EC number  

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
586Phosphoserine.
588Phosphoserine.

Sequence Information
Sequence length633
Molecular weight67572

	---------+----------+----------+----------+----------+
MGLLNSSDKE IIKRALPKAS NKIIDVTVAR LYIAYPDKNE WQYTGLSGAL   50
ALVDDLVGNT FFLKLVDING HRGVIWDQEL YVNFEYYQDR TFFHTFEMEE   100
CFAGLLFVDI NEASHFLKRV QKRERYANRK TLLNKNAVAL TKKVREEQKS   150
QVVHGPRGES LIDNQRKRYN YEDVDTIPTT KHKAPPPPPP TAETFDSDQT   200
SSFSDINSTT ASAPTTPAPA LPPASPEVRK EETHPKHSLP PLPNQFAPLP   250
DPPQHNSPPQ NNAPSQPQSN PFPFPIPEIP STQSATNPFP FPVPQQQFNQ   300
APSMGIPQQN RPLPQLPNRN NRPVPPPPPM RTTTEGSGVR LPAPPPPPRR   350
GPAPPPPPHR HVTSNTLNSA GGNSLLPQAT GRRGPAPPPP PRASRPTPNV   400
TMQQNPQQYN NSNRPFGYQT NSNMSSPPPP PVTTFNTLTP QMTAATGQPA   450
VPLPQNTQAP SQATNVPVAP PPPPASLGQS QIPQSAPSAP IPPTLPSTTS   500
AAPPPPPAFL TQQPQSGGAP APPPPPQMPA TSTSGGGSFA ETTGDAGRDA   550
LLASIRGAGG IGALRKVDKS QLDKPSVLLQ EARGESASPP AAAGNGGTPG   600
GPPASLADAL AAALNKRKTK VGAHDDMDNG DDW                     633