SCUD (Saccharomyces Cerevisiae Ubiquitination Database)


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Substrate Information

General Information
Standard nameKSP1
Systematic nameYHR082C
DescriptionSerine/threonine-protein kinase KSP1

Ubiquitination Features
NumberSiteTypeE2E3 ClassE3 SubclassSubstrate ReceptorUbiquitin ReceptorDUBEffect
1         
* Blank: not determined, NA: not applicable.

References
[1]EvidencePurification & Protein ID
Methodpurification (6xHis-Myc-ubiquitin), LC/MS, validation (virtual Western blot)
Reference"Systematic approach for validating the ubiquitinated proteome."
Seyfried NT, Xu P, Duong DM, Cheng D, Hanfelt J, Peng J.
Anal Chem. 80(11):4161-9 (2008) [18433149]

Cross References
Swiss-Prot accessionP38691
SGD YHR082C
Gene Ontology (GO)
Cellular componentGO:0005634nucleusIDA:SGD.
Molecular functionGO:0004674protein serine/threonine kinase activityIDA:SGD.
Biological processGO:0006468protein amino acid phosphorylationIDA:SGD.
EC number 2.7.11.1

Additional Features
Other Post Translational Modifications (PTM)
Residue indexType
416Phosphoserine.
526Phosphothreonine.
529Phosphoserine.
591Phosphoserine.
646Phosphoserine.
827Phosphoserine.
845Phosphoserine.
970Phosphoserine.
973Phosphoserine.

Sequence Information
Sequence length1029
Molecular weight117082

	---------+----------+----------+----------+----------+
MTLDYEIYKE GGILNNRYQK IEDISEGSYG YVSLAKDVRE KRLVAVKYIF   50
KLEDDGQYDG PQDDENDCDS SDCDDDEDTK VDTDRHENEN GNASSNNGSS   100
REKKHNLYKH KKSLISSKVK SRLSNNICLE AMYEVDIQTK IGRHQNIAAL   150
LDFFDSYIIM EYCSGGDLYE AIKADAVPKK TKSITHIITQ IMDAIEYVHN   200
KGIYHRDIKP ENILISGIDW TIKLTDWGLA TTDKTSMDRN VGSERYMSPE   250
LFDSNLDIKE RKEPYDCAKV DLWAMGIVFL NIVFHKNPFS IANQSDKSFC   300
YFAANREALF DVFSTMAYDF FQVLRYSLTI DPANRDLKMM RTELQNLSEY   350
TLDDEYYNNL DEGYEETMID GLPPQPVPPS SAPVSLPTPI SSSNKQHMPE   400
FKKDFNFNNV NERKRSDVSQ NQNVASGFFK KPSTQQQKFF NQGYNTTLST   450
HERAKSAPKF KFKKRNKYGR TDNQFSKPVN IEDRKKSKIL KKSRKPLGIP   500
TPNTHMNNFF HDYKARDEFN TRDFFTPPSV QHRYMEGFSN NNNKQYRQNR   550
NYNNNNNNSN NNHGSNYNNF NNGNSYIKGW NKNFNKYRRP SSSSYTGKSP   600
LSRYNMSYNH NNNSSINGYA RRGSTTTVQH SPGAYIPPNA RNHHVSPTNQ   650
FLRVPQSTAP DISTVLGGKP SYQEHYTQDS MDSEGDHDSD DVLFTLEEGD   700
HDFVNGMDNL SINDHLPHTT VGSHNEVFVH ASTNHNNNGN NNHIDTNSTT   750
NQYHRQYIPP PLTTSLHINN NNNESNELPD LLKSPASSEA HLNLSSGPID   800
PILTGNIGNR YSHSSDSKEL EQERRLSMEQ KFKNGVYVPP HHRKSFNLGT   850
QVPPMNMKTS NEATLSVSHN SVNFGGSYNS RRSSANESNP LHMNKALEKL   900
SSSPGAKSSF VGFPKPLLPR NHSSTTIALQ NEDVFADSNN DAIIFEDEEY   950
EGESDKMAHG KMEGGDNESS STSPDERQIF GPYEIYAQTF AGSTHDKKLG   1000
AGRKTSIQDE MVGSLEQYKN NWLILQQQD                          1029